1w27
From Proteopedia
(New page: 200px<br /> <applet load="1w27" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w27, resolution 1.70Å" /> '''PHENYLALANINE AMMON...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1W27 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Petroselinum_crispum Petroselinum crispum]] with DTT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.5 4.3.1.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W27 OCA]]. | + | 1W27 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Petroselinum_crispum Petroselinum crispum]] with DTT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Phenylalanine_ammonia-lyase Phenylalanine ammonia-lyase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.5 4.3.1.5]]. Structure known Active Site: MI1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W27 OCA]]. |
==Reference== | ==Reference== | ||
Structural basis for the entrance into the phenylpropanoid metabolism catalyzed by phenylalanine ammonia-lyase., Ritter H, Schulz GE, Plant Cell. 2004 Dec;16(12):3426-36. Epub 2004 Nov 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15548745 15548745] | Structural basis for the entrance into the phenylpropanoid metabolism catalyzed by phenylalanine ammonia-lyase., Ritter H, Schulz GE, Plant Cell. 2004 Dec;16(12):3426-36. Epub 2004 Nov 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15548745 15548745] | ||
[[Category: Petroselinum crispum]] | [[Category: Petroselinum crispum]] | ||
| + | [[Category: Phenylalanine ammonia-lyase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ritter, H.]] | [[Category: Ritter, H.]] | ||
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[[Category: phenylpropanoid metabolism]] | [[Category: phenylpropanoid metabolism]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:32:47 2007'' |
Revision as of 11:28, 30 October 2007
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PHENYLALANINE AMMONIA-LYASE (PAL) FROM PETROSELINUM CRISPUM
Overview
Because of its key role in secondary phenylpropanoid metabolism, Phe, ammonia-lyase is one of the most extensively studied plant enzymes. To, provide a basis for detailed structure-function studies, the enzyme from, parsley (Petroselinum crispum) was crystallized, and the structure was, elucidated at 1.7-A resolution. It contains the unusual electrophilic, 4-methylidene-imidazole-5-one group, which is derived from a tripeptide, segment in two autocatalytic dehydration reactions. The enzyme resembles, His ammonia-lyase from the general His degradation pathway but contains, 207 additional residues, mainly in an N-terminal extension rigidifying a, domain interface and in an inserted alpha-helical domain restricting the, access to the active center. Presumably, Phe ammonia-lyase developed ... [(full description)]
About this Structure
1W27 is a [Single protein] structure of sequence from [Petroselinum crispum] with DTT as [ligand]. Active as [Phenylalanine ammonia-lyase], with EC number [4.3.1.5]. Structure known Active Site: MI1. Full crystallographic information is available from [OCA].
Reference
Structural basis for the entrance into the phenylpropanoid metabolism catalyzed by phenylalanine ammonia-lyase., Ritter H, Schulz GE, Plant Cell. 2004 Dec;16(12):3426-36. Epub 2004 Nov 17. PMID:15548745
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