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1w2a
From Proteopedia
(New page: 200px<br /> <applet load="1w2a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w2a, resolution 2.51Å" /> '''DEACETOXYCEPHALOSPO...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1W2A is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with FE and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.20.1 1.14.20.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W2A OCA]]. | + | 1W2A is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with FE and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Deacetoxycephalosporin-C_synthase Deacetoxycephalosporin-C synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.20.1 1.14.20.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W2A OCA]]. |
==Reference== | ==Reference== | ||
Conformational flexibility of the C terminus with implications for substrate binding and catalysis revealed in a new crystal form of deacetoxycephalosporin C synthase., Oster LM, van Scheltinga AC, Valegard K, Hose AM, Dubus A, Hajdu J, Andersson I, J Mol Biol. 2004 Oct 8;343(1):157-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15381427 15381427] | Conformational flexibility of the C terminus with implications for substrate binding and catalysis revealed in a new crystal form of deacetoxycephalosporin C synthase., Oster LM, van Scheltinga AC, Valegard K, Hose AM, Dubus A, Hajdu J, Andersson I, J Mol Biol. 2004 Oct 8;343(1):157-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15381427 15381427] | ||
| + | [[Category: Deacetoxycephalosporin-C synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces clavuligerus]] | [[Category: Streptomyces clavuligerus]] | ||
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[[Category: penicillin]] | [[Category: penicillin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:33:45 2007'' |
Revision as of 11:29, 30 October 2007
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DEACETOXYCEPHALOSPORIN C SYNTHASE (WITH HIS-TAG) COMPLEXED WITH FE(II) AND ETHYLENE GLYCOL
Overview
Deacetoxycephalosporin C synthase (DAOCS) from Streptomyces clavuligerus, catalyses the oxidative ring expansion of the penicillin nucleus into the, nucleus of cephalosporins. The reaction requires dioxygen and, 2-oxoglutarate as co-substrates to create a reactive iron-oxygen, intermediate from a ferrous iron in the active site. The active enzyme is, monomeric in solution. The structure of DAOCS was determined earlier from, merohedrally twinned crystals where the last four C-terminal residues, (308-311) of one molecule penetrate the active site of a neighbouring, molecule, creating a cyclic trimeric structure in the crystal. Shortening, the polypeptide chain from the C terminus by more than four residues, diminishes activity. Here, we describe a new crystal form of DAOCS in, which trimer ... [(full description)]
About this Structure
1W2A is a [Single protein] structure of sequence from [Streptomyces clavuligerus] with FE and EDO as [ligands]. Active as [Deacetoxycephalosporin-C synthase], with EC number [1.14.20.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Conformational flexibility of the C terminus with implications for substrate binding and catalysis revealed in a new crystal form of deacetoxycephalosporin C synthase., Oster LM, van Scheltinga AC, Valegard K, Hose AM, Dubus A, Hajdu J, Andersson I, J Mol Biol. 2004 Oct 8;343(1):157-71. PMID:15381427
Page seeded by OCA on Tue Oct 30 13:33:45 2007
Categories: Deacetoxycephalosporin-C synthase | Single protein | Streptomyces clavuligerus | Andersson, I. | Dubus, A. | Hajdu, J. | Hose, A.Mackenzie. | Oster, L.M. | Scheltinga, A.C.Terwisscha.Van. | Valegard, K. | EDO | FE | 2-oxoglutarate dependent oxygenases | Cephalosporin | Mononuclear ferrous enzymes | Oxidoreductase | Penicillin
