3eq3
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:3eq3.png|left|200px]] | [[Image:3eq3.png|left|200px]] | ||
| - | <!-- | ||
| - | The line below this paragraph, containing "STRUCTURE_3eq3", creates the "Structure Box" on the page. | ||
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet) | ||
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| - | or leave the SCENE parameter empty for the default display. | ||
| - | --> | ||
{{STRUCTURE_3eq3| PDB=3eq3 | SCENE= }} | {{STRUCTURE_3eq3| PDB=3eq3 | SCENE= }} | ||
===Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM=== | ===Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM=== | ||
| - | |||
| - | <!-- | ||
| - | The line below this paragraph, {{ABSTRACT_PUBMED_19020518}}, adds the Publication Abstract to the page | ||
| - | (as it appears on PubMed at http://www.pubmed.gov), where 19020518 is the PubMed ID number. | ||
| - | --> | ||
{{ABSTRACT_PUBMED_19020518}} | {{ABSTRACT_PUBMED_19020518}} | ||
Revision as of 11:36, 25 July 2012
Contents |
Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM
The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of the kirromycin-stalled ribosome bound with ternary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI). The three maps suggest a common binding manner of cognate aa-tRNAs in their specific binding with both the ribosome and EF-Tu. All three aa-tRNAs have the same 'loaded spring' conformation with a kink and twist between the D-stem and anticodon stem. The three complexes are similarly integrated in an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-binding CCA end of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the accuracy of aa-tRNA selection.
Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM., Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J, EMBO J. 2008 Dec 17;27(24):3322-31. Epub 2008 Nov 20. PMID:19020518
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3eq3 is a 9 chain structure of Ribosomal protein L11 and Ribosomal protein S12 with sequence from Escherichia coli k12. Full crystallographic information is available from OCA.
See Also
Reference
- Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J. Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM. EMBO J. 2008 Dec 17;27(24):3322-31. Epub 2008 Nov 20. PMID:19020518 doi:10.1038/emboj.2008.243
Categories: Escherichia coli k12 | Agirrezabala, X. | Frank, J. | Li, W. | A/t-trna | Antibiotic resistance | Automated data collection | Elongation factor | Gtp-binding | Membrane | Methylation | Nucleotide-binding | Phosphoprotein | Protein biosynthesis | Protein translation | Ribonucleoprotein | Ribosomal protein | Ribosomal protein-rna complex | Rna-binding | Rrna-binding | Ternary complex | Trna-binding
