1ap8
From Proteopedia
(New page: 200px<br /><applet load="1ap8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ap8" /> '''TRANSLATION INITIATION FACTOR EIF4E IN COMPL...) |
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| - | [[Image:1ap8.gif|left|200px]]<br /><applet load="1ap8" size=" | + | [[Image:1ap8.gif|left|200px]]<br /><applet load="1ap8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ap8" /> | caption="1ap8" /> | ||
'''TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES'''<br /> | '''TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | eIF4E, the mRNA cap binding protein, is a master switch that controls | + | eIF4E, the mRNA cap binding protein, is a master switch that controls eukaryotic translation. To be active, it must bind eIF4G and form the eIF4F complex, which also contains eIF4A. Translation is downregulated by association of eIF4E with 4E-BP, which occupies the eIF4G binding site. Signalling events acting on 4E-BP cause it to dissociate from eIF4E, and eIF4E is then free to bind eIF4G to form the active eIF4F complex. We have solved the structure of the yeast eIF4E/m7Gpp complex in a CHAPS micelle. We determined the position of the second nucleotide in a complex with m7GpppA, and identified the 4E-BP binding site. eIF4E has a curved eight-stranded antiparallel beta-sheet, decorated with three helices on the convex face and three smaller helices inserted in connecting loops. The m7G of the cap is intercalated into a stack of tryptophans in the concave face. The 4E-BP binding site is located in a region encompassing one edge of the beta-sheet, the adjacent helix a2 and several regions of non-regular secondary structure. It is adjacent to, but does not overlap the cap-binding site. |
==About this Structure== | ==About this Structure== | ||
| - | 1AP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with M7G as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1AP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=M7G:'>M7G</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Fletcher, M.]] | [[Category: Fletcher, M.]] | ||
| - | [[Category: Gingras, A | + | [[Category: Gingras, A C.]] |
[[Category: Li, H.]] | [[Category: Li, H.]] | ||
[[Category: Matsuo, H.]] | [[Category: Matsuo, H.]] | ||
| - | [[Category: Mcguire, A | + | [[Category: Mcguire, A M.]] |
[[Category: Sonenberg, N.]] | [[Category: Sonenberg, N.]] | ||
[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
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[[Category: translation initiation factor]] | [[Category: translation initiation factor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:46:52 2008'' |
Revision as of 09:46, 21 February 2008
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TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES
Overview
eIF4E, the mRNA cap binding protein, is a master switch that controls eukaryotic translation. To be active, it must bind eIF4G and form the eIF4F complex, which also contains eIF4A. Translation is downregulated by association of eIF4E with 4E-BP, which occupies the eIF4G binding site. Signalling events acting on 4E-BP cause it to dissociate from eIF4E, and eIF4E is then free to bind eIF4G to form the active eIF4F complex. We have solved the structure of the yeast eIF4E/m7Gpp complex in a CHAPS micelle. We determined the position of the second nucleotide in a complex with m7GpppA, and identified the 4E-BP binding site. eIF4E has a curved eight-stranded antiparallel beta-sheet, decorated with three helices on the convex face and three smaller helices inserted in connecting loops. The m7G of the cap is intercalated into a stack of tryptophans in the concave face. The 4E-BP binding site is located in a region encompassing one edge of the beta-sheet, the adjacent helix a2 and several regions of non-regular secondary structure. It is adjacent to, but does not overlap the cap-binding site.
About this Structure
1AP8 is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of translation factor eIF4E bound to m7GDP and interaction with 4E-binding protein., Matsuo H, Li H, McGuire AM, Fletcher CM, Gingras AC, Sonenberg N, Wagner G, Nat Struct Biol. 1997 Sep;4(9):717-24. PMID:9302999
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