1aro
From Proteopedia
(New page: 200px<br /><applet load="1aro" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aro, resolution 2.8Å" /> '''T7 RNA POLYMERASE COM...) |
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| - | [[Image:1aro.jpg|left|200px]]<br /><applet load="1aro" size=" | + | [[Image:1aro.jpg|left|200px]]<br /><applet load="1aro" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1aro, resolution 2.8Å" /> | caption="1aro, resolution 2.8Å" /> | ||
'''T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME'''<br /> | '''T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The T7 RNA polymerase-T7 lysozyme complex regulates phage gene expression | + | The T7 RNA polymerase-T7 lysozyme complex regulates phage gene expression during infection of Escherichia coli. The 2.8 A crystal structure of the complex reveals that lysozyme binds at a site remote from the polymerase active site, suggesting an indirect mechanism of inhibition. Comparison of the T7 RNA polymerase structure with that of the homologous pol I family of DNA polymerases reveals identities in the catalytic site but also differences specific to RNA polymerase function. The structure of T7 RNA polymerase presented here differs significantly from a previously published structure. Sequence similarities between phage RNA polymerases and those from mitochondria and chloroplasts, when interpreted in the context of our revised model of T7 RNA polymerase, suggest a conserved fold. |
==About this Structure== | ==About this Structure== | ||
| - | 1ARO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 1ARO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:47:34 2008'' |
Revision as of 09:47, 21 February 2008
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T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME
Overview
The T7 RNA polymerase-T7 lysozyme complex regulates phage gene expression during infection of Escherichia coli. The 2.8 A crystal structure of the complex reveals that lysozyme binds at a site remote from the polymerase active site, suggesting an indirect mechanism of inhibition. Comparison of the T7 RNA polymerase structure with that of the homologous pol I family of DNA polymerases reveals identities in the catalytic site but also differences specific to RNA polymerase function. The structure of T7 RNA polymerase presented here differs significantly from a previously published structure. Sequence similarities between phage RNA polymerases and those from mitochondria and chloroplasts, when interpreted in the context of our revised model of T7 RNA polymerase, suggest a conserved fold.
About this Structure
1ARO is a Protein complex structure of sequences from Bacteriophage t7 with as ligand. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme., Jeruzalmi D, Steitz TA, EMBO J. 1998 Jul 15;17(14):4101-13. PMID:9670025
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