1au6
From Proteopedia
(New page: 200px<br /><applet load="1au6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1au6" /> '''SOLUTION STRUCTURE OF DNA D(CATGCATG) INTERS...) |
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| - | [[Image:1au6.gif|left|200px]]<br /><applet load="1au6" size=" | + | [[Image:1au6.gif|left|200px]]<br /><applet load="1au6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1au6" /> | caption="1au6" /> | ||
'''SOLUTION STRUCTURE OF DNA D(CATGCATG) INTERSTRAND-CROSSLINKED BY BISPLATIN COMPOUND (1,1/T,T), NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''SOLUTION STRUCTURE OF DNA D(CATGCATG) INTERSTRAND-CROSSLINKED BY BISPLATIN COMPOUND (1,1/T,T), NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The bifunctional platinum compound, [(trans-PtCI(NH)3)2)2(H2N(CH2)4NH2)]2+, forms a stable adduct with the | + | The bifunctional platinum compound, [(trans-PtCI(NH)3)2)2(H2N(CH2)4NH2)]2+, forms a stable adduct with the self-complementary DNA oligomer CATGCATG, with the two platinum atoms coordinated at the N7 positions of the two symmetrical G4 nucleotides. The NMR-derived structure shows that the DNA octamer forms a novel hairpin structure with the platinated G4 residue adopting a syn conformation and the guanine base in the minor groove. Two such hairpins stack end-over-end and are linked together by the butanediamine tether to form a dumbbell structure. Such unusual structural distortion is different from that of the anticancer drug cisplatin-DNA adduct and may provide clues to explain the distinct biological activities of the two compounds. |
==About this Structure== | ==About this Structure== | ||
| - | 1AU6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with BPT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1AU6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=BPT:'>BPT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AU6 OCA]. |
==Reference== | ==Reference== | ||
A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA., Yang D, van Boom SS, Reedijk J, van Boom JH, Farrell N, Wang AH, Nat Struct Biol. 1995 Jul;2(7):577-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7664126 7664126] | A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA., Yang D, van Boom SS, Reedijk J, van Boom JH, Farrell N, Wang AH, Nat Struct Biol. 1995 Jul;2(7):577-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7664126 7664126] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Boom, J | + | [[Category: Boom, J H.Van.]] |
| - | [[Category: Boom, S | + | [[Category: Boom, S S.G E.Van.]] |
[[Category: Farrell, N.]] | [[Category: Farrell, N.]] | ||
[[Category: Reedijk, J.]] | [[Category: Reedijk, J.]] | ||
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
[[Category: Yang, D.]] | [[Category: Yang, D.]] | ||
[[Category: BPT]] | [[Category: BPT]] | ||
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[[Category: t)]] | [[Category: t)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:17 2008'' |
Revision as of 09:48, 21 February 2008
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SOLUTION STRUCTURE OF DNA D(CATGCATG) INTERSTRAND-CROSSLINKED BY BISPLATIN COMPOUND (1,1/T,T), NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The bifunctional platinum compound, [(trans-PtCI(NH)3)2)2(H2N(CH2)4NH2)]2+, forms a stable adduct with the self-complementary DNA oligomer CATGCATG, with the two platinum atoms coordinated at the N7 positions of the two symmetrical G4 nucleotides. The NMR-derived structure shows that the DNA octamer forms a novel hairpin structure with the platinated G4 residue adopting a syn conformation and the guanine base in the minor groove. Two such hairpins stack end-over-end and are linked together by the butanediamine tether to form a dumbbell structure. Such unusual structural distortion is different from that of the anticancer drug cisplatin-DNA adduct and may provide clues to explain the distinct biological activities of the two compounds.
About this Structure
1AU6 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA., Yang D, van Boom SS, Reedijk J, van Boom JH, Farrell N, Wang AH, Nat Struct Biol. 1995 Jul;2(7):577-8. PMID:7664126
Page seeded by OCA on Thu Feb 21 11:48:17 2008
