1avq
From Proteopedia
(New page: 200px<br /><applet load="1avq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1avq, resolution 2.4Å" /> '''TOROIDAL STRUCTURE OF...) |
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| - | [[Image:1avq.gif|left|200px]]<br /><applet load="1avq" size=" | + | [[Image:1avq.gif|left|200px]]<br /><applet load="1avq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1avq, resolution 2.4Å" /> | caption="1avq, resolution 2.4Å" /> | ||
'''TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS'''<br /> | '''TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Structure determination at 2.4 angstrom resolution shows that | + | Structure determination at 2.4 angstrom resolution shows that lambda-exonuclease consists of three subunits that form a toroid. The central channel is funnel shaped, tapering from an inner diameter of about 30 angstroms at the wider end to 15 angstroms at the narrow end. This is adequate to accommodate the DNA substrate and thus provides a structural basis for the ability of the enzyme to sequentially hydrolyze thousands of nucleotides in a highly processive manner. The results also suggest the locations of the active sites and the constraints that limit cleavage to a single strand. |
==About this Structure== | ==About this Structure== | ||
| - | 1AVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda] with PO4 and ACT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_(lambda-induced) Exodeoxyribonuclease (lambda-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.3 3.1.11.3] Full crystallographic information is available from [http:// | + | 1AVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_(lambda-induced) Exodeoxyribonuclease (lambda-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.3 3.1.11.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Exodeoxyribonuclease (lambda-induced)]] | [[Category: Exodeoxyribonuclease (lambda-induced)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kovall, R | + | [[Category: Kovall, R A.]] |
| - | [[Category: Matthews, B | + | [[Category: Matthews, B W.]] |
[[Category: ACT]] | [[Category: ACT]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
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[[Category: dna recombination and repair]] | [[Category: dna recombination and repair]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:46 2008'' |
Revision as of 09:48, 21 February 2008
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TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS
Overview
Structure determination at 2.4 angstrom resolution shows that lambda-exonuclease consists of three subunits that form a toroid. The central channel is funnel shaped, tapering from an inner diameter of about 30 angstroms at the wider end to 15 angstroms at the narrow end. This is adequate to accommodate the DNA substrate and thus provides a structural basis for the ability of the enzyme to sequentially hydrolyze thousands of nucleotides in a highly processive manner. The results also suggest the locations of the active sites and the constraints that limit cleavage to a single strand.
About this Structure
1AVQ is a Single protein structure of sequence from Enterobacteria phage lambda with and as ligands. Active as Exodeoxyribonuclease (lambda-induced), with EC number 3.1.11.3 Full crystallographic information is available from OCA.
Reference
Toroidal structure of lambda-exonuclease., Kovall R, Matthews BW, Science. 1997 Sep 19;277(5333):1824-7. PMID:9295273
Page seeded by OCA on Thu Feb 21 11:48:46 2008
