1aw6
From Proteopedia
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==Overview== | ==Overview== | ||
| - | We have refined the solution structure of cadmium-bound GAL4 and present | + | We have refined the solution structure of cadmium-bound GAL4 and present its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/- 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to 3J(113Cd,1H) are complex. |
==About this Structure== | ==About this Structure== | ||
| - | 1AW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure | + | 1AW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 125D. Known structural/functional Sites: <scene name='pdbsite=CD4:Cd+Binding+Site'>CD4</scene> and <scene name='pdbsite=CD5:Cd+Binding+Site'>CD5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AW6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Baleja, J | + | [[Category: Baleja, J D.]] |
[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
[[Category: CD]] | [[Category: CD]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:53 2008'' |
Revision as of 09:48, 21 February 2008
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GAL4 (CD), NMR, 24 STRUCTURES
Overview
We have refined the solution structure of cadmium-bound GAL4 and present its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/- 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to 3J(113Cd,1H) are complex.
About this Structure
1AW6 is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. This structure supersedes the now removed PDB entry 125D. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination., Baleja JD, Thanabal V, Wagner G, J Biomol NMR. 1997 Dec;10(4):397-401. PMID:9460244
Page seeded by OCA on Thu Feb 21 11:48:53 2008
