1aw2
From Proteopedia
(New page: 200px<br /><applet load="1aw2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aw2, resolution 2.65Å" /> '''TRIOSEPHOSPHATE ISOM...) |
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| - | [[Image:1aw2.gif|left|200px]]<br /><applet load="1aw2" size=" | + | [[Image:1aw2.gif|left|200px]]<br /><applet load="1aw2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1aw2, resolution 2.65Å" /> | caption="1aw2, resolution 2.65Å" /> | ||
'''TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS'''<br /> | '''TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The purification and characterization of triose-phosphate isomerase from | + | The purification and characterization of triose-phosphate isomerase from the psychrophilic bacterium Vibrio marinus (vTIM) is described. Crystal structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate complex (at a 2.7-A resolution) are also presented. The optimal growth temperature of Vibrio marinus is 15 degrees C. Stability studies show that vTIM is an unstable protein with a half-life of only 10 min at 25 degrees C. The vTIM sequence is most closely related to the sequence of Escherichia coli TIM (eTIM) (66% identity), and several unique structural features described for eTIM are also seen in vTIM, but eTIM is considerably more stable. The Td values of vTIM and eTIM, determined by calorimetric studies, are 41 and 54 degrees C, respectively. Amino acid sequence comparison reveals that vTIM has an alanine in loop 8 (at position 238), whereas all other TIM sequences known to date have a serine. The vTIM mutant A238S was produced and characterized. Compared with wild type, the catalytic efficiency of the A238S mutant is somewhat reduced, and its stability is considerably increased. |
==About this Structure== | ==About this Structure== | ||
| - | 1AW2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moritella_marina Moritella marina] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] Full crystallographic information is available from [http:// | + | 1AW2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moritella_marina Moritella marina] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AW2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Triose-phosphate isomerase]] | [[Category: Triose-phosphate isomerase]] | ||
[[Category: Maes, D.]] | [[Category: Maes, D.]] | ||
| - | [[Category: Wierenga, R | + | [[Category: Wierenga, R K.]] |
| - | [[Category: Zeelen, J | + | [[Category: Zeelen, J P.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: isomerase]] | [[Category: isomerase]] | ||
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[[Category: vibrio marinus]] | [[Category: vibrio marinus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:52 2008'' |
Revision as of 09:48, 21 February 2008
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TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS
Overview
The purification and characterization of triose-phosphate isomerase from the psychrophilic bacterium Vibrio marinus (vTIM) is described. Crystal structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate complex (at a 2.7-A resolution) are also presented. The optimal growth temperature of Vibrio marinus is 15 degrees C. Stability studies show that vTIM is an unstable protein with a half-life of only 10 min at 25 degrees C. The vTIM sequence is most closely related to the sequence of Escherichia coli TIM (eTIM) (66% identity), and several unique structural features described for eTIM are also seen in vTIM, but eTIM is considerably more stable. The Td values of vTIM and eTIM, determined by calorimetric studies, are 41 and 54 degrees C, respectively. Amino acid sequence comparison reveals that vTIM has an alanine in loop 8 (at position 238), whereas all other TIM sequences known to date have a serine. The vTIM mutant A238S was produced and characterized. Compared with wild type, the catalytic efficiency of the A238S mutant is somewhat reduced, and its stability is considerably increased.
About this Structure
1AW2 is a Single protein structure of sequence from Moritella marina with as ligand. Active as Triose-phosphate isomerase, with EC number 5.3.1.1 Full crystallographic information is available from OCA.
Reference
Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties., Alvarez M, Zeelen JP, Mainfroid V, Rentier-Delrue F, Martial JA, Wyns L, Wierenga RK, Maes D, J Biol Chem. 1998 Jan 23;273(4):2199-206. PMID:9442062
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