1b0a
From Proteopedia
(New page: 200px<br /><applet load="1b0a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b0a, resolution 2.56Å" /> '''5,10, METHYLENE-TETR...) |
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| - | [[Image:1b0a.jpg|left|200px]]<br /><applet load="1b0a" size=" | + | [[Image:1b0a.jpg|left|200px]]<br /><applet load="1b0a" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1b0a, resolution 2.56Å" /> | caption="1b0a, resolution 2.56Å" /> | ||
'''5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.'''<br /> | '''5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of a bifunctional 5,10-methylene-tetrahydrofolate | + | The structure of a bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase from Escherichia coli has been determined at 2.5 A resolution in the absence of bound substrates and compared to the NADP-bound structure of the homologous enzyme domains from a trifunctional human synthetase enzyme. Superposition of these structures allows the identification of a highly conserved cluster of basic residues that are appropriately positioned to serve as a binding site for the poly-gamma-glutamyl tail of the tetrahydrofolate substrate. Modeling studies and molecular dynamic simulations of bound methylene-tetrahydrofolate and NADP shows that this binding site would allow interaction of the nicotinamide and pterin rings in the dehydrogenase active site. Comparison of these enzymes also indicates differences between their active sites that might allow the development of inhibitors specific to the bacterial target. |
==About this Structure== | ==About this Structure== | ||
| - | 1B0A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1B0A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B0A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ari, L | + | [[Category: Ari, L D.]] |
[[Category: Dyer, D.]] | [[Category: Dyer, D.]] | ||
| - | [[Category: Huang, J | + | [[Category: Huang, J Y.]] |
[[Category: Rabinowitz, J.]] | [[Category: Rabinowitz, J.]] | ||
| - | [[Category: Shen, B | + | [[Category: Shen, B W.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: bifunctional]] | [[Category: bifunctional]] | ||
[[Category: channeling]] | [[Category: channeling]] | ||
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[[Category: folate]] | [[Category: folate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:50:08 2008'' |
Revision as of 09:50, 21 February 2008
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5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.
Overview
The structure of a bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase from Escherichia coli has been determined at 2.5 A resolution in the absence of bound substrates and compared to the NADP-bound structure of the homologous enzyme domains from a trifunctional human synthetase enzyme. Superposition of these structures allows the identification of a highly conserved cluster of basic residues that are appropriately positioned to serve as a binding site for the poly-gamma-glutamyl tail of the tetrahydrofolate substrate. Modeling studies and molecular dynamic simulations of bound methylene-tetrahydrofolate and NADP shows that this binding site would allow interaction of the nicotinamide and pterin rings in the dehydrogenase active site. Comparison of these enzymes also indicates differences between their active sites that might allow the development of inhibitors specific to the bacterial target.
About this Structure
1B0A is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The crystal structure of a bacterial, bifunctional 5,10 methylene-tetrahydrofolate dehydrogenase/cyclohydrolase., Shen BW, Dyer DH, Huang JY, D'Ari L, Rabinowitz J, Stoddard BL, Protein Sci. 1999 Jun;8(6):1342-9. PMID:10386884
Page seeded by OCA on Thu Feb 21 11:50:08 2008
