2c5b
From Proteopedia
(New page: 200px<br /> <applet load="2c5b" size="450" color="white" frame="true" align="right" spinBox="true" caption="2c5b, resolution 2.50Å" /> '''X-RAY CRYSTAL STRUC...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2C5B is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]] with MET and 5F1 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C5B OCA]]. | + | 2C5B is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]] with MET and 5F1 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Adenosyl-fluoride_synthase Adenosyl-fluoride synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C5B OCA]]. |
==Reference== | ==Reference== | ||
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16604208 16604208] | Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16604208 16604208] | ||
| + | [[Category: Adenosyl-fluoride synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces cattleya]] | [[Category: Streptomyces cattleya]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:37:30 2007'' |
Revision as of 11:32, 30 October 2007
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X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.
Overview
The fluorinase enzyme from Streptomyces cattleya displays an unusual, ability in biocatalysis in that it forms a C-F bond. We now report that, the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to, accommodate this substrate series. It emerges from this study that the, enzyme does not require a planar ribose conformation of the substrate to, catalyse C-F bond formation.
About this Structure
2C5B is a [Single protein] structure of sequence from [Streptomyces cattleya] with MET and 5F1 as [ligands]. Active as [Adenosyl-fluoride synthase], with EC number [2.5.1.63]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208
Page seeded by OCA on Tue Oct 30 13:37:30 2007
