1b7g
From Proteopedia
(New page: 200px<br /><applet load="1b7g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b7g, resolution 2.05Å" /> '''GLYCERALDEHYDE 3-PHO...) |
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| - | [[Image:1b7g.jpg|left|200px]]<br /><applet load="1b7g" size=" | + | [[Image:1b7g.jpg|left|200px]]<br /><applet load="1b7g" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1b7g, resolution 2.05Å" /> | caption="1b7g, resolution 2.05Å" /> | ||
'''GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE'''<br /> | '''GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the | + | The enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the archaea shows low sequence identity (16-20%) with its eubacterial and eukaryotic counterparts. The crystal structure of the apo GAPDH from Sulfolobus solfataricus has been determined by multiple isomorphous replacement at 2.05 A resolution. The enzyme has several differences in secondary structure when compared with eubacterial GAPDHs, with an overall increase in the number of alpha-helices. There is a relocation of the active-site residues within the catalytic domain of the enzyme. The thermostability of the S. solfataricus enzyme can be attributed to a combination of an ion pair cluster and an intrasubunit disulphide bond. |
==About this Structure== | ==About this Structure== | ||
| - | 1B7G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] Full crystallographic information is available from [http:// | + | 1B7G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7G OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
| - | [[Category: Isupov, M | + | [[Category: Isupov, M N.]] |
| - | [[Category: Littlechild, J | + | [[Category: Littlechild, J A.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: archaea; hyperthermophile; gapdh; hyperthermophilic dehydrogenase]] | [[Category: archaea; hyperthermophile; gapdh; hyperthermophilic dehydrogenase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:52:20 2008'' |
Revision as of 09:52, 21 February 2008
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GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE
Overview
The enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the archaea shows low sequence identity (16-20%) with its eubacterial and eukaryotic counterparts. The crystal structure of the apo GAPDH from Sulfolobus solfataricus has been determined by multiple isomorphous replacement at 2.05 A resolution. The enzyme has several differences in secondary structure when compared with eubacterial GAPDHs, with an overall increase in the number of alpha-helices. There is a relocation of the active-site residues within the catalytic domain of the enzyme. The thermostability of the S. solfataricus enzyme can be attributed to a combination of an ion pair cluster and an intrasubunit disulphide bond.
About this Structure
1B7G is a Single protein structure of sequence from Sulfolobus solfataricus with as ligand. Active as Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating), with EC number 1.2.1.12 Full crystallographic information is available from OCA.
Reference
Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus., Isupov MN, Fleming TM, Dalby AR, Crowhurst GS, Bourne PC, Littlechild JA, J Mol Biol. 1999 Aug 20;291(3):651-60. PMID:10448043
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