1bfe
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Modular PDZ domains, found in many cell junction-associated proteins, mediate the clustering of membrane ion channels by binding to their | + | Modular PDZ domains, found in many cell junction-associated proteins, mediate the clustering of membrane ion channels by binding to their C-terminus. The X-ray crystallographic structures of the third PDZ domain from the synaptic protein PSD-95 in complex with and in the absence of its peptide ligand have been determined at 1.8 angstroms and 2.3 angstroms resolution, respectively. The structures reveal that a four-residue C-terminal stretch (X-Thr/Ser-X-Val-COO(-)) engages the PDZ domain through antiparallel main chain interactions with a beta sheet of the domain. Recognition of the terminal carboxylate group of the peptide is conferred by a cradle of main chain amides provided by a Gly-Leu-Gly-Phe loop as well as by an arginine side chain. Specific side chain interactions and a prominent hydrophobic pocket explain the selective recognition of the C-terminal consensus sequence. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Doyle, D | + | [[Category: Doyle, D A.]] |
[[Category: Kim, E.]] | [[Category: Kim, E.]] | ||
[[Category: Lee, A.]] | [[Category: Lee, A.]] | ||
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[[Category: protein localization]] | [[Category: protein localization]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:40 2008'' |
Revision as of 09:54, 21 February 2008
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THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95
Overview
Modular PDZ domains, found in many cell junction-associated proteins, mediate the clustering of membrane ion channels by binding to their C-terminus. The X-ray crystallographic structures of the third PDZ domain from the synaptic protein PSD-95 in complex with and in the absence of its peptide ligand have been determined at 1.8 angstroms and 2.3 angstroms resolution, respectively. The structures reveal that a four-residue C-terminal stretch (X-Thr/Ser-X-Val-COO(-)) engages the PDZ domain through antiparallel main chain interactions with a beta sheet of the domain. Recognition of the terminal carboxylate group of the peptide is conferred by a cradle of main chain amides provided by a Gly-Leu-Gly-Phe loop as well as by an arginine side chain. Specific side chain interactions and a prominent hydrophobic pocket explain the selective recognition of the C-terminal consensus sequence.
About this Structure
1BFE is a Single protein structure of sequence from Rattus norvegicus. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ., Doyle DA, Lee A, Lewis J, Kim E, Sheng M, MacKinnon R, Cell. 1996 Jun 28;85(7):1067-76. PMID:8674113
Page seeded by OCA on Thu Feb 21 11:54:40 2008
