1bf4
From Proteopedia
(New page: 200px<br /><applet load="1bf4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bf4, resolution 1.6Å" /> '''CHROMOSOMAL DNA-BINDI...) |
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| - | [[Image:1bf4.gif|left|200px]]<br /><applet load="1bf4" size=" | + | [[Image:1bf4.gif|left|200px]]<br /><applet load="1bf4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bf4, resolution 1.6Å" /> | caption="1bf4, resolution 1.6Å" /> | ||
'''CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX'''<br /> | '''CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus | + | Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus solfataricus and S. acidocaldarius respectively. These proteins have high thermal, acid and chemical stability. They bind DNA without marked sequence preference and increase the Tm of DNA by approximately 40 degrees C. Sso7d in complex with GTAATTAC and GCGT(iU)CGC + GCGAACGC was crystallized in different crystal lattices and the crystal structures were solved at high resolution. Sso7d binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 60 degrees) by the intercalation of the hydrophobic side chains of Val 26 and Met 29. The intercalation sites are different in the two complexes. Observations of this novel DNA binding mode in three independent crystal lattices indicate that it is not a function of crystal packing. |
==About this Structure== | ==About this Structure== | ||
| - | 1BF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http:// | + | 1BF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BF4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus acidocaldarius]] | [[Category: Sulfolobus acidocaldarius]] | ||
| - | [[Category: Gao, Y | + | [[Category: Gao, Y G.]] |
[[Category: Lim, L.]] | [[Category: Lim, L.]] | ||
[[Category: Padmanabhan, S.]] | [[Category: Padmanabhan, S.]] | ||
[[Category: Robinson, H.]] | [[Category: Robinson, H.]] | ||
| - | [[Category: Shriver, J | + | [[Category: Shriver, J W.]] |
[[Category: Su, S.]] | [[Category: Su, S.]] | ||
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
[[Category: archaebacteria]] | [[Category: archaebacteria]] | ||
[[Category: complex (dna-binding protein/dna)]] | [[Category: complex (dna-binding protein/dna)]] | ||
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[[Category: hyperthermophile]] | [[Category: hyperthermophile]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:36 2008'' |
Revision as of 09:54, 21 February 2008
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CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX
Overview
Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus solfataricus and S. acidocaldarius respectively. These proteins have high thermal, acid and chemical stability. They bind DNA without marked sequence preference and increase the Tm of DNA by approximately 40 degrees C. Sso7d in complex with GTAATTAC and GCGT(iU)CGC + GCGAACGC was crystallized in different crystal lattices and the crystal structures were solved at high resolution. Sso7d binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 60 degrees) by the intercalation of the hydrophobic side chains of Val 26 and Met 29. The intercalation sites are different in the two complexes. Observations of this novel DNA binding mode in three independent crystal lattices indicate that it is not a function of crystal packing.
About this Structure
1BF4 is a Single protein structure of sequence from Sulfolobus acidocaldarius. Full crystallographic information is available from OCA.
Reference
The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA., Gao YG, Su SY, Robinson H, Padmanabhan S, Lim L, McCrary BS, Edmondson SP, Shriver JW, Wang AH, Nat Struct Biol. 1998 Sep;5(9):782-6. PMID:9731772
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