1bfr
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Crystals of E. coli cytochrome b1, alias bacterioferritin, were grown fr | + | Crystals of E. coli cytochrome b1, alias bacterioferritin, were grown fr om a low ionic strength solution. The resulting monoclniic P21 structure was solved by molecular replacement and refined using noncrystallographi c symmetries applied to the fundamental unit, consisting of two protein subunits and a single haem. From the Patterson self-rotation results it was shown that the asymmetric unit of the monoclinic crystal consists of 12 such dimers and corresponds to a complete, nearly spherical, molecule of bacterioferritin (M4 = 450 kDa) of 432 point-group symmetry. It is thus the most symmetrical cytochrome. As previously determined for the tetragonal form, the haem is located in a special position on a local twofold axis of the dimer. A bimetal centre is also observed within the four-helix bundle of each monomer; a metal-binding site is located on the fourfold axis. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Dautant, A.]] | [[Category: Dautant, A.]] | ||
[[Category: Frolow, F.]] | [[Category: Frolow, F.]] | ||
| - | [[Category: Gilboa, A | + | [[Category: Gilboa, A J.Kalb.]] |
| - | [[Category: Meyer, J | + | [[Category: Meyer, J B.]] |
[[Category: Precigoux, G.]] | [[Category: Precigoux, G.]] | ||
| - | [[Category: Sweet, R | + | [[Category: Sweet, R M.]] |
[[Category: Yariv, J.]] | [[Category: Yariv, J.]] | ||
[[Category: HEM]] | [[Category: HEM]] | ||
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[[Category: iron storage]] | [[Category: iron storage]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:55 2008'' |
Revision as of 09:54, 21 February 2008
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IRON STORAGE AND ELECTRON TRANSPORT
Overview
Crystals of E. coli cytochrome b1, alias bacterioferritin, were grown fr om a low ionic strength solution. The resulting monoclniic P21 structure was solved by molecular replacement and refined using noncrystallographi c symmetries applied to the fundamental unit, consisting of two protein subunits and a single haem. From the Patterson self-rotation results it was shown that the asymmetric unit of the monoclinic crystal consists of 12 such dimers and corresponds to a complete, nearly spherical, molecule of bacterioferritin (M4 = 450 kDa) of 432 point-group symmetry. It is thus the most symmetrical cytochrome. As previously determined for the tetragonal form, the haem is located in a special position on a local twofold axis of the dimer. A bimetal centre is also observed within the four-helix bundle of each monomer; a metal-binding site is located on the fourfold axis.
About this Structure
1BFR is a Single protein structure of sequence from Escherichia coli with and as ligands. Known structural/functional Sites: , , , , , , , , , , , , , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
Structure of a monoclinic crystal from of cyctochrome b1 (Bacterioferritin) from E. coli., Dautant A, Meyer JB, Yariv J, Precigoux G, Sweet RM, Kalb AJ, Frolow F, Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):16-24. PMID:9867433
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