1bjf
From Proteopedia
(New page: 200px<br /><applet load="1bjf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bjf, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1bjf.jpg|left|200px]]<br /><applet load="1bjf" size=" | + | [[Image:1bjf.jpg|left|200px]]<br /><applet load="1bjf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bjf, resolution 2.4Å" /> | caption="1bjf, resolution 2.4Å" /> | ||
'''CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS'''<br /> | '''CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of calcium-bound unmyristoylated bovine neurocalcin | + | The crystal structure of calcium-bound unmyristoylated bovine neurocalcin from Escherichia coli has been determined at 2.4 A resolution. The three-dimensional structure reveals a highly compact structure consisting of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii) a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that is disabled from calcium-binding due to a Cys-Pro sequence in the Ca2+-binding loop. The crystal structure of neurocalcin resembles photoreceptor recoverin in overall topology, however its EF2- and EF4-hands differ. Recently, neurocalcin in the calcium-bound state has been shown to stimulate mammalian rod outer segment membrane guanylate cyclase. A possible site for cyclase activity based on the three-dimensional structure is discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kumar, V | + | [[Category: Kumar, V D.]] |
[[Category: Vijay-Kumar, S.]] | [[Category: Vijay-Kumar, S.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: neuronal specific guanylate cyclase activator]] | [[Category: neuronal specific guanylate cyclase activator]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:55:56 2008'' |
Revision as of 09:55, 21 February 2008
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CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS
Overview
The crystal structure of calcium-bound unmyristoylated bovine neurocalcin from Escherichia coli has been determined at 2.4 A resolution. The three-dimensional structure reveals a highly compact structure consisting of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii) a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that is disabled from calcium-binding due to a Cys-Pro sequence in the Ca2+-binding loop. The crystal structure of neurocalcin resembles photoreceptor recoverin in overall topology, however its EF2- and EF4-hands differ. Recently, neurocalcin in the calcium-bound state has been shown to stimulate mammalian rod outer segment membrane guanylate cyclase. A possible site for cyclase activity based on the three-dimensional structure is discussed.
About this Structure
1BJF is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of recombinant bovine neurocalcin., Vijay-Kumar S, Kumar VD, Nat Struct Biol. 1999 Jan;6(1):80-8. PMID:9886296
Page seeded by OCA on Thu Feb 21 11:55:56 2008
