1bjt
From Proteopedia
(New page: 200px<br /><applet load="1bjt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bjt, resolution 2.5Å" /> '''TOPOISOMERASE II RESI...) |
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| - | [[Image:1bjt.gif|left|200px]]<br /><applet load="1bjt" size=" | + | [[Image:1bjt.gif|left|200px]]<br /><applet load="1bjt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bjt, resolution 2.5Å" /> | caption="1bjt, resolution 2.5Å" /> | ||
'''TOPOISOMERASE II RESIDUES 409-1201'''<br /> | '''TOPOISOMERASE II RESIDUES 409-1201'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Type II DNA topoisomerases mediate the passage of one DNA duplex through a | + | Type II DNA topoisomerases mediate the passage of one DNA duplex through a transient break in another, an event essential for chromosome segregation and cell viability. The active sites of the type II topoisomerase dimer associate covalently with the DNA break-points and must separate by at least the width of the second DNA duplex to accommodate transport. A new structure of the Saccharomyces cerevisiae topoisomerase II DNA-binding and cleavage core suggests that in addition to conformational changes in the DNA-opening platform, a dramatic reorganization of accessory domains may occur during catalysis. These conformational differences have implications for both the DNA-breaking and duplex-transport events in the topo II reaction mechanism, suggest a mechanism by which two distinct drug-resistance loci interact, and illustrate the scope of structural changes in the cycling of molecular machines. |
==About this Structure== | ==About this Structure== | ||
| - | 1BJT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] Full crystallographic information is available from [http:// | + | 1BJT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berger, J | + | [[Category: Berger, J M.]] |
| - | [[Category: Bogden, C | + | [[Category: Bogden, C E.]] |
[[Category: Fass, D.]] | [[Category: Fass, D.]] | ||
[[Category: dna topology]] | [[Category: dna topology]] | ||
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[[Category: topoisomerase]] | [[Category: topoisomerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:07 2008'' |
Revision as of 09:56, 21 February 2008
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TOPOISOMERASE II RESIDUES 409-1201
Overview
Type II DNA topoisomerases mediate the passage of one DNA duplex through a transient break in another, an event essential for chromosome segregation and cell viability. The active sites of the type II topoisomerase dimer associate covalently with the DNA break-points and must separate by at least the width of the second DNA duplex to accommodate transport. A new structure of the Saccharomyces cerevisiae topoisomerase II DNA-binding and cleavage core suggests that in addition to conformational changes in the DNA-opening platform, a dramatic reorganization of accessory domains may occur during catalysis. These conformational differences have implications for both the DNA-breaking and duplex-transport events in the topo II reaction mechanism, suggest a mechanism by which two distinct drug-resistance loci interact, and illustrate the scope of structural changes in the cycling of molecular machines.
About this Structure
1BJT is a Single protein structure of sequence from Saccharomyces cerevisiae. Active as DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3 Full crystallographic information is available from OCA.
Reference
Quaternary changes in topoisomerase II may direct orthogonal movement of two DNA strands., Fass D, Bogden CE, Berger JM, Nat Struct Biol. 1999 Apr;6(4):322-6. PMID:10201398
Page seeded by OCA on Thu Feb 21 11:56:07 2008
