3boi
From Proteopedia
m (Protected "3boi" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
[[Image:3boi.png|left|200px]] | [[Image:3boi.png|left|200px]] | ||
| - | <!-- | ||
| - | The line below this paragraph, containing "STRUCTURE_3boi", creates the "Structure Box" on the page. | ||
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet) | ||
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| - | or leave the SCENE parameter empty for the default display. | ||
| - | --> | ||
{{STRUCTURE_3boi| PDB=3boi | SCENE= }} | {{STRUCTURE_3boi| PDB=3boi | SCENE= }} | ||
===Snow Flea Antifreeze Protein Racemate=== | ===Snow Flea Antifreeze Protein Racemate=== | ||
| - | |||
| - | <!-- | ||
| - | The line below this paragraph, {{ABSTRACT_PUBMED_18598029}}, adds the Publication Abstract to the page | ||
| - | (as it appears on PubMed at http://www.pubmed.gov), where 18598029 is the PubMed ID number. | ||
| - | --> | ||
{{ABSTRACT_PUBMED_18598029}} | {{ABSTRACT_PUBMED_18598029}} | ||
| Line 22: | Line 11: | ||
==See Also== | ==See Also== | ||
| - | *[[Antifreeze protein]] | + | *[[Antifreeze protein|Antifreeze protein]] |
==Reference== | ==Reference== | ||
Revision as of 18:57, 25 July 2012
Contents |
Snow Flea Antifreeze Protein Racemate
Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal formation from a racemic solution containing equal amounts of the chemically synthesized proteins d-sfAFP and l-sfAFP occurred much more readily than for l-sfAFP alone. More facile crystal formation also occurred from a quasi-racemic mixture of d-sfAFP and l-Se-sfAFP, a chemical protein analogue that contains an additional -SeCH2- moiety at one residue and thus differs slightly from the true enantiomer. Multiple wavelength anomalous dispersion (MAD) phasing from quasi-racemate crystals was then used to determine the X-ray structure of the sfAFP protein molecule. The resulting model was used to solve by molecular replacement the X-ray structure of l-sfAFP to a resolution of 0.98 A. The l-sfAFP molecule is made up of six antiparallel left-handed PPII helixes, stacked in two sets of three, to form a compact brick-like structure with one hydrophilic face and one hydrophobic face. This is a novel experimental protein structure and closely resembles a structural model proposed for sfAFP. These results illustrate the utility of total chemical synthesis combined with racemic crystallization and X-ray crystallography for determining the unknown structure of a protein.
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers., Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB, J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:18598029
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3boi is a 2 chain structure of Antifreeze protein. Full crystallographic information is available from OCA.
See Also
Reference
- Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB. X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers. J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:18598029 doi:http://dx.doi.org/10.1021/ja8013538
