1bnk
From Proteopedia
(New page: 200px<br /> <applet load="1bnk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bnk, resolution 2.7Å" /> '''HUMAN 3-METHYLADENIN...) |
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| - | [[Image:1bnk.gif|left|200px]]<br /> | + | [[Image:1bnk.gif|left|200px]]<br /><applet load="1bnk" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1bnk, resolution 2.7Å" /> | caption="1bnk, resolution 2.7Å" /> | ||
'''HUMAN 3-METHYLADENINE DNA GLYCOSYLASE COMPLEXED TO DNA'''<br /> | '''HUMAN 3-METHYLADENINE DNA GLYCOSYLASE COMPLEXED TO DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | DNA N-glycosylases are base excision-repair proteins that locate and | + | DNA N-glycosylases are base excision-repair proteins that locate and cleave damaged bases from DNA as the first step in restoring the genetic blueprint. The human enzyme 3-methyladenine DNA glycosylase removes a diverse group of damaged bases from DNA, including cytotoxic and mutagenic alkylation adducts of purines. We report the crystal structure of human 3-methyladenine DNA glycosylase complexed to a mechanism-based pyrrolidine inhibitor. The enzyme has intercalated into the minor groove of DNA, causing the abasic pyrrolidine nucleotide to flip into the enzyme active site, where a bound water is poised for nucleophilic attack. The structure shows an elegant means of exposing a nucleotide for base excision as well as a network of residues that could catalyze the in-line displacement of a damaged base from the phosphodeoxyribose backbone. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BNK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http:// | + | 1BNK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ellenberger, T.]] | [[Category: Ellenberger, T.]] | ||
| - | [[Category: Lau, A | + | [[Category: Lau, A Y.]] |
[[Category: Samson, L.]] | [[Category: Samson, L.]] | ||
| - | [[Category: Schaerer, O | + | [[Category: Schaerer, O D.]] |
| - | [[Category: Verdine, G | + | [[Category: Verdine, G L.]] |
[[Category: dna glycosylase]] | [[Category: dna glycosylase]] | ||
[[Category: dna repair]] | [[Category: dna repair]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:57:05 2008'' |
Revision as of 09:57, 21 February 2008
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HUMAN 3-METHYLADENINE DNA GLYCOSYLASE COMPLEXED TO DNA
Contents |
Overview
DNA N-glycosylases are base excision-repair proteins that locate and cleave damaged bases from DNA as the first step in restoring the genetic blueprint. The human enzyme 3-methyladenine DNA glycosylase removes a diverse group of damaged bases from DNA, including cytotoxic and mutagenic alkylation adducts of purines. We report the crystal structure of human 3-methyladenine DNA glycosylase complexed to a mechanism-based pyrrolidine inhibitor. The enzyme has intercalated into the minor groove of DNA, causing the abasic pyrrolidine nucleotide to flip into the enzyme active site, where a bound water is poised for nucleophilic attack. The structure shows an elegant means of exposing a nucleotide for base excision as well as a network of residues that could catalyze the in-line displacement of a damaged base from the phosphodeoxyribose backbone.
Disease
Known disease associated with this structure: Opitz G syndrome, type I OMIM:[300552]
About this Structure
1BNK is a Single protein structure of sequence from Homo sapiens. Active as DNA-3-methyladenine glycosylase II, with EC number 3.2.2.21 Full crystallographic information is available from OCA.
Reference
Crystal structure of a human alkylbase-DNA repair enzyme complexed to DNA: mechanisms for nucleotide flipping and base excision., Lau AY, Scharer OD, Samson L, Verdine GL, Ellenberger T, Cell. 1998 Oct 16;95(2):249-58. PMID:9790531
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