1bp4
From Proteopedia
(New page: 200px<br /><applet load="1bp4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bp4, resolution 2.2Å" /> '''USE OF PAPAIN AS A MO...) |
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| - | [[Image:1bp4.gif|left|200px]]<br /><applet load="1bp4" size=" | + | [[Image:1bp4.gif|left|200px]]<br /><applet load="1bp4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bp4, resolution 2.2Å" /> | caption="1bp4, resolution 2.2Å" /> | ||
'''USE OF PAPAIN AS A MODEL FOR THE STRUCTURE-BASED DESIGN OF CATHEPSIN K INHIBITORS. CRYSTAL STRUCTURES OF TWO PAPAIN INHIBITOR COMPLEXES DEMONSTRATE BINDING TO S'-SUBSITES.'''<br /> | '''USE OF PAPAIN AS A MODEL FOR THE STRUCTURE-BASED DESIGN OF CATHEPSIN K INHIBITORS. CRYSTAL STRUCTURES OF TWO PAPAIN INHIBITOR COMPLEXES DEMONSTRATE BINDING TO S'-SUBSITES.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Papain has been used as a surrogate enzyme in a drug design effort to | + | Papain has been used as a surrogate enzyme in a drug design effort to obtain potent and selective inhibitors of cathepsin K, a new member of the papain superfamily of cysteine proteases that is selectively and highly expressed in osteoclasts and is implicated in bone resorption. Here we report the crystal structures of two papain-inhibitor complexes and the rational design of novel cathepsin K inhibitors. Unlike previously known crystal structures of papain-inhibitor complexes, our papain structures show ligand binding extending deep within the S'-subsites. The two inhibitor complexes, carbobenzyloxyleucinyl-leucinyl-leucinal and carbobenzyloxy-L-leucinyl-L-leucinyl methoxymethyl ketone, were refined to 2.2- and 2.5-A resolution with R-factors of 0.190 and 0. 217, respectively. The S'-subsite interactions with the inhibitors are dominated by an aromatic-aromatic stacking and an oxygen-aromatic ring edge interaction. The knowledge of S'-subsite interactions led to a design strategy for an inhibitor spanning both subsites and yielded a novel, symmetric inhibitor selective for cathepsin K. Simultaneous exploitation of both S- and S'-sites provides a general strategy for the design of cysteine protease inhibitors having high specificity to their target enzymes. |
==About this Structure== | ==About this Structure== | ||
| - | 1BP4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya] with ALD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1BP4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya] with <scene name='pdbligand=ALD:'>ALD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BP4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Carica papaya]] | [[Category: Carica papaya]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Abdel-Mequid, S | + | [[Category: Abdel-Mequid, S S.]] |
| - | [[Category: Carr, T | + | [[Category: Carr, T J.]] |
| - | [[Category: Desjarlais, R | + | [[Category: Desjarlais, R L.]] |
| - | [[Category: Janson, C | + | [[Category: Janson, C A.]] |
| - | [[Category: Lalonde, J | + | [[Category: Lalonde, J M.]] |
| - | [[Category: Smith, W | + | [[Category: Smith, W W.]] |
| - | [[Category: Thompson, S | + | [[Category: Thompson, S K.]] |
| - | [[Category: Tomaszek, T | + | [[Category: Tomaszek, T A.]] |
| - | [[Category: Veber, D | + | [[Category: Veber, D F.]] |
| - | [[Category: Yamashita, D | + | [[Category: Yamashita, D S.]] |
[[Category: Zhao, B.]] | [[Category: Zhao, B.]] | ||
[[Category: ALD]] | [[Category: ALD]] | ||
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[[Category: sulfhydryl proteinase]] | [[Category: sulfhydryl proteinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:57:36 2008'' |
Revision as of 09:57, 21 February 2008
|
USE OF PAPAIN AS A MODEL FOR THE STRUCTURE-BASED DESIGN OF CATHEPSIN K INHIBITORS. CRYSTAL STRUCTURES OF TWO PAPAIN INHIBITOR COMPLEXES DEMONSTRATE BINDING TO S'-SUBSITES.
Overview
Papain has been used as a surrogate enzyme in a drug design effort to obtain potent and selective inhibitors of cathepsin K, a new member of the papain superfamily of cysteine proteases that is selectively and highly expressed in osteoclasts and is implicated in bone resorption. Here we report the crystal structures of two papain-inhibitor complexes and the rational design of novel cathepsin K inhibitors. Unlike previously known crystal structures of papain-inhibitor complexes, our papain structures show ligand binding extending deep within the S'-subsites. The two inhibitor complexes, carbobenzyloxyleucinyl-leucinyl-leucinal and carbobenzyloxy-L-leucinyl-L-leucinyl methoxymethyl ketone, were refined to 2.2- and 2.5-A resolution with R-factors of 0.190 and 0. 217, respectively. The S'-subsite interactions with the inhibitors are dominated by an aromatic-aromatic stacking and an oxygen-aromatic ring edge interaction. The knowledge of S'-subsite interactions led to a design strategy for an inhibitor spanning both subsites and yielded a novel, symmetric inhibitor selective for cathepsin K. Simultaneous exploitation of both S- and S'-sites provides a general strategy for the design of cysteine protease inhibitors having high specificity to their target enzymes.
About this Structure
1BP4 is a Single protein structure of sequence from Carica papaya with as ligand. Full crystallographic information is available from OCA.
Reference
Use of papain as a model for the structure-based design of cathepsin K inhibitors: crystal structures of two papain-inhibitor complexes demonstrate binding to S'-subsites., LaLonde JM, Zhao B, Smith WW, Janson CA, DesJarlais RL, Tomaszek TA, Carr TJ, Thompson SK, Oh HJ, Yamashita DS, Veber DF, Abdel-Meguid SS, J Med Chem. 1998 Nov 5;41(23):4567-76. PMID:9804696
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