1bp7
From Proteopedia
(New page: 200px<br /><applet load="1bp7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bp7, resolution 3.00Å" /> '''GROUP I MOBILE INTRO...) |
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| - | [[Image:1bp7.gif|left|200px]]<br /><applet load="1bp7" size=" | + | [[Image:1bp7.gif|left|200px]]<br /><applet load="1bp7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bp7, resolution 3.00Å" /> | caption="1bp7, resolution 3.00Å" /> | ||
'''GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA'''<br /> | '''GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI | + | The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation. |
==About this Structure== | ==About this Structure== | ||
| - | 1BP7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1BP7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BP7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Junior, R | + | [[Category: Junior, R J.Monnat.]] |
| - | [[Category: Jurica, M | + | [[Category: Jurica, M S.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: chloroplast dna]] | [[Category: chloroplast dna]] | ||
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[[Category: laglidadg motif]] | [[Category: laglidadg motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:57:37 2008'' |
Revision as of 09:57, 21 February 2008
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GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA
Overview
The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.
About this Structure
1BP7 is a Single protein structure of sequence from Chlamydomonas reinhardtii with as ligand. Full crystallographic information is available from OCA.
Reference
DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI., Jurica MS, Monnat RJ Jr, Stoddard BL, Mol Cell. 1998 Oct;2(4):469-76. PMID:9809068
Page seeded by OCA on Thu Feb 21 11:57:37 2008
