1br1
From Proteopedia
(New page: 200px<br /> <applet load="1br1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1br1, resolution 3.5Å" /> '''SMOOTH MUSCLE MYOSIN...) |
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| - | [[Image:1br1.gif|left|200px]]<br /> | + | [[Image:1br1.gif|left|200px]]<br /><applet load="1br1" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1br1" size=" | + | |
caption="1br1, resolution 3.5Å" /> | caption="1br1, resolution 3.5Å" /> | ||
'''SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.ALF4 BOUND AT THE ACTIVE SITE'''<br /> | '''SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.ALF4 BOUND AT THE ACTIVE SITE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structures of an expressed vertebrate smooth muscle myosin | + | The crystal structures of an expressed vertebrate smooth muscle myosin motor domain (MD) and a motor domain-essential light chain (ELC) complex (MDE), both with a transition state analog (MgADP x AIF4-) in the active site, have been determined to 2.9 A and 3.5 A resolution, respectively. The MDE structure with an ATP analog (MgADP x BeFx) was also determined to 3.6 A resolution. In all three structures, a domain of the C-terminal region, the "converter," is rotated approximately 70 degrees from that in nucleotide-free skeletal subfragment 1 (S1). We have found that the MDE-BeFx and MDE-AIF4- structures are almost identical, consistent with the fact that they both bind weakly to actin. A comparison of the lever arm positions in MDE-AIF4- and in nucleotide-free skeletal S1 shows that a potential displacement of approximately 10 nm can be achieved during the power stroke. |
==About this Structure== | ==About this Structure== | ||
| - | 1BR1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with MG, ALF and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1BR1 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb18_1.html Myosin]]. Active as [http://en.wikipedia.org/wiki/Myosin_ATPase Myosin ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.1 3.6.4.1] Full crystallographic information is available from [http:// | + | 1BR1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ALF:'>ALF</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1BR1 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb18_1.html Myosin]]. Active as [http://en.wikipedia.org/wiki/Myosin_ATPase Myosin ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.1 3.6.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BR1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cohen, C.]] | [[Category: Cohen, C.]] | ||
[[Category: Dominguez, R.]] | [[Category: Dominguez, R.]] | ||
| - | [[Category: Trybus, K | + | [[Category: Trybus, K M.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: ALF]] | [[Category: ALF]] | ||
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[[Category: muscle protein]] | [[Category: muscle protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:09 2008'' |
Revision as of 09:58, 21 February 2008
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SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.ALF4 BOUND AT THE ACTIVE SITE
Overview
The crystal structures of an expressed vertebrate smooth muscle myosin motor domain (MD) and a motor domain-essential light chain (ELC) complex (MDE), both with a transition state analog (MgADP x AIF4-) in the active site, have been determined to 2.9 A and 3.5 A resolution, respectively. The MDE structure with an ATP analog (MgADP x BeFx) was also determined to 3.6 A resolution. In all three structures, a domain of the C-terminal region, the "converter," is rotated approximately 70 degrees from that in nucleotide-free skeletal subfragment 1 (S1). We have found that the MDE-BeFx and MDE-AIF4- structures are almost identical, consistent with the fact that they both bind weakly to actin. A comparison of the lever arm positions in MDE-AIF4- and in nucleotide-free skeletal S1 shows that a potential displacement of approximately 10 nm can be achieved during the power stroke.
About this Structure
1BR1 is a Protein complex structure of sequences from Gallus gallus with , and as ligands. The following page contains interesting information on the relation of 1BR1 with [Myosin]. Active as Myosin ATPase, with EC number 3.6.4.1 Full crystallographic information is available from OCA.
Reference
Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state., Dominguez R, Freyzon Y, Trybus KM, Cohen C, Cell. 1998 Sep 4;94(5):559-71. PMID:9741621
Page seeded by OCA on Thu Feb 21 11:58:09 2008
Categories: Gallus gallus | Myosin | Myosin ATPase | Protein complex | Cohen, C. | Dominguez, R. | Trybus, K M. | ADP | ALF | MG | Muscle protein
