1br0
From Proteopedia
(New page: 200px<br /><applet load="1br0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1br0" /> '''THREE DIMENSIONAL STRUCTURE OF THE N-TERMINA...) |
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| - | [[Image:1br0.gif|left|200px]]<br /><applet load="1br0" size=" | + | [[Image:1br0.gif|left|200px]]<br /><applet load="1br0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1br0" /> | caption="1br0" /> | ||
'''THREE DIMENSIONAL STRUCTURE OF THE N-TERMINAL DOMAIN OF SYNTAXIN 1A'''<br /> | '''THREE DIMENSIONAL STRUCTURE OF THE N-TERMINAL DOMAIN OF SYNTAXIN 1A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Syntaxin 1A plays a central role in neurotransmitter release through | + | Syntaxin 1A plays a central role in neurotransmitter release through multiple protein-protein interactions. We have used NMR spectroscopy to identify an autonomously folded N-terminal domain in syntaxin 1A and to elucidate its three-dimensional structure. This 120-residue N-terminal domain is conserved in plasma membrane syntaxins but not in other syntaxins, indicating a specific role in exocytosis. The domain contains three long alpha helices that form an up-and-down bundle with a left-handed twist. A striking residue conservation is observed throughout a long groove that is likely to provide a specific surface for protein-protein interactions. A highly acidic region binds to the C2A domain of synaptotagmin I in a Ca2+-dependent interaction that may serve as an electrostatic switch in neurotransmitter release. |
==About this Structure== | ==About this Structure== | ||
| - | 1BR0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | + | 1BR0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BR0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Fernandez, I.]] | [[Category: Fernandez, I.]] | ||
[[Category: Rizo, J.]] | [[Category: Rizo, J.]] | ||
| - | [[Category: Sudhof, T | + | [[Category: Sudhof, T C.]] |
[[Category: Ubach, J.]] | [[Category: Ubach, J.]] | ||
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
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[[Category: syntaxin]] | [[Category: syntaxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:09 2008'' |
Revision as of 09:58, 21 February 2008
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THREE DIMENSIONAL STRUCTURE OF THE N-TERMINAL DOMAIN OF SYNTAXIN 1A
Overview
Syntaxin 1A plays a central role in neurotransmitter release through multiple protein-protein interactions. We have used NMR spectroscopy to identify an autonomously folded N-terminal domain in syntaxin 1A and to elucidate its three-dimensional structure. This 120-residue N-terminal domain is conserved in plasma membrane syntaxins but not in other syntaxins, indicating a specific role in exocytosis. The domain contains three long alpha helices that form an up-and-down bundle with a left-handed twist. A striking residue conservation is observed throughout a long groove that is likely to provide a specific surface for protein-protein interactions. A highly acidic region binds to the C2A domain of synaptotagmin I in a Ca2+-dependent interaction that may serve as an electrostatic switch in neurotransmitter release.
About this Structure
1BR0 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of an evolutionarily conserved N-terminal domain of syntaxin 1A., Fernandez I, Ubach J, Dulubova I, Zhang X, Sudhof TC, Rizo J, Cell. 1998 Sep 18;94(6):841-9. PMID:9753330
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