1e0x

From Proteopedia

Revision as of 11:36, 30 October 2007

XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A

Overview

Endoxylanases are a group of enzymes that hydrolyze the beta-1, 4-linked, xylose backbone of xylans. They are predominantly found in two discrete, sequence families known as glycoside hydrolase families 10 and 11. The, Streptomyces lividans xylanase Xyl10A is a family 10 enzyme, the native, structure of which has previously been determined by x-ray crystallography, at a 2.6 A resolution (Derewenda, U., Swenson, L., Green, R., Wei, Y., Morosoli, R., Shareck, F., Kluepfel, D., and Derewenda, Z. S. (1994) J., Biol. Chem. 269, 20811-20814). Here, we report the native structure of, Xyl10A refined at a resolution of 1.2 A, which reveals many features such, as the rare occurrence of a discretely disordered disulfide bond between, residues Cys-168 and Cys-201. In order to investigate substrate ... [(full description)]

About this Structure

1E0X is a [Single protein] structure of sequence from [Streptomyces lividans] with GOL as [ligand]. Active as [Endo-1,4-beta-xylanase], with EC number [3.2.1.8]. Structure known Active Sites: AC1, AC2, AC3, AC4, ACA, ACB, NUA and NUB. Full crystallographic information is available from [OCA].

Reference

Substrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10A., Ducros V, Charnock SJ, Derewenda U, Derewenda ZS, Dauter Z, Dupont C, Shareck F, Morosoli R, Kluepfel D, Davies GJ, J Biol Chem. 2000 Jul 28;275(30):23020-6. PMID:10930426

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