1btc
From Proteopedia
(New page: 200px<br /><applet load="1btc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1btc, resolution 2.0Å" /> '''THREE-DIMENSIONAL STR...) |
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| - | [[Image:1btc.gif|left|200px]]<br /><applet load="1btc" size=" | + | [[Image:1btc.gif|left|200px]]<br /><applet load="1btc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1btc, resolution 2.0Å" /> | caption="1btc, resolution 2.0Å" /> | ||
'''THREE-DIMENSIONAL STRUCTURE OF SOYBEAN BETA-AMYLASE DETERMINED AT 3.0 ANGSTROMS RESOLUTION: PRELIMINARY CHAIN TRACING OF THE COMPLEX WITH ALPHA-CYCLODEXTRIN'''<br /> | '''THREE-DIMENSIONAL STRUCTURE OF SOYBEAN BETA-AMYLASE DETERMINED AT 3.0 ANGSTROMS RESOLUTION: PRELIMINARY CHAIN TRACING OF THE COMPLEX WITH ALPHA-CYCLODEXTRIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of a complex of soybean beta-amylase [EC | + | The three-dimensional structure of a complex of soybean beta-amylase [EC 3.2.1.2] with an inhibitor, alpha-cyclodextrin, has been determined at 3.0 A resolution by X-ray diffraction analysis. Preliminary chain tracing showed that the enzyme folded into large and small domains. The large domain has a (beta alpha)8 super-secondary structure, while the smaller one is formed from two long loops extending from the beta 3 and beta 4 strands of the (beta alpha)8 structure. The interface of the two domains together with shorter loops from the (beta alpha)8 structure form a deep cleft, in which alpha-cyclodextrin binds slightly away from the center. Two maltose molecules also bind in the cleft. One shares a binding site with alpha-cyclodextrin and the other is situated more deeply in the cleft. |
==About this Structure== | ==About this Structure== | ||
| - | 1BTC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SO4 and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2] Full crystallographic information is available from [http:// | + | 1BTC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BTC OCA]. |
==Reference== | ==Reference== | ||
| - | Three-dimensional structure of soybean beta-amylase determined at 3.0 A resolution: preliminary chain tracing of the complex with alpha-cyclodextrin., Mikami B, Sato M, Shibata T, Hirose M, Aibara S, Katsube Y, Morita Y, J Biochem | + | Three-dimensional structure of soybean beta-amylase determined at 3.0 A resolution: preliminary chain tracing of the complex with alpha-cyclodextrin., Mikami B, Sato M, Shibata T, Hirose M, Aibara S, Katsube Y, Morita Y, J Biochem. 1992 Oct;112(4):541-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1491009 1491009] |
[[Category: Beta-amylase]] | [[Category: Beta-amylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hehre, E | + | [[Category: Hehre, E J.]] |
[[Category: Hirose, M.]] | [[Category: Hirose, M.]] | ||
[[Category: Katsube, Y.]] | [[Category: Katsube, Y.]] | ||
[[Category: Mikami, B.]] | [[Category: Mikami, B.]] | ||
[[Category: Morita, Y.]] | [[Category: Morita, Y.]] | ||
| - | [[Category: Sacchettini, J | + | [[Category: Sacchettini, J C.]] |
[[Category: Sato, M.]] | [[Category: Sato, M.]] | ||
[[Category: BME]] | [[Category: BME]] | ||
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[[Category: hydrolase(o-glycosyl)]] | [[Category: hydrolase(o-glycosyl)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:52 2008'' |
Revision as of 09:58, 21 February 2008
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THREE-DIMENSIONAL STRUCTURE OF SOYBEAN BETA-AMYLASE DETERMINED AT 3.0 ANGSTROMS RESOLUTION: PRELIMINARY CHAIN TRACING OF THE COMPLEX WITH ALPHA-CYCLODEXTRIN
Overview
The three-dimensional structure of a complex of soybean beta-amylase [EC 3.2.1.2] with an inhibitor, alpha-cyclodextrin, has been determined at 3.0 A resolution by X-ray diffraction analysis. Preliminary chain tracing showed that the enzyme folded into large and small domains. The large domain has a (beta alpha)8 super-secondary structure, while the smaller one is formed from two long loops extending from the beta 3 and beta 4 strands of the (beta alpha)8 structure. The interface of the two domains together with shorter loops from the (beta alpha)8 structure form a deep cleft, in which alpha-cyclodextrin binds slightly away from the center. Two maltose molecules also bind in the cleft. One shares a binding site with alpha-cyclodextrin and the other is situated more deeply in the cleft.
About this Structure
1BTC is a Single protein structure of sequence from [1] with and as ligands. Active as Beta-amylase, with EC number 3.2.1.2 Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of soybean beta-amylase determined at 3.0 A resolution: preliminary chain tracing of the complex with alpha-cyclodextrin., Mikami B, Sato M, Shibata T, Hirose M, Aibara S, Katsube Y, Morita Y, J Biochem. 1992 Oct;112(4):541-6. PMID:1491009
Page seeded by OCA on Thu Feb 21 11:58:52 2008
Categories: Beta-amylase | Single protein | Hehre, E J. | Hirose, M. | Katsube, Y. | Mikami, B. | Morita, Y. | Sacchettini, J C. | Sato, M. | BME | SO4 | Hydrolase(o-glycosyl)
