1be4
From Proteopedia
(New page: 200px<br /> <applet load="1be4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1be4, resolution 2.4Å" /> '''NUCLEOSIDE DIPHOSPHA...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1BE4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with PCG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BE4 OCA]]. | + | 1BE4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with PCG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6]]. Structure known Active Site: NUL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BE4 OCA]]. |
==Reference== | ==Reference== | ||
Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure., Abdulaev NG, Karaschuk GN, Ladner JE, Kakuev DL, Yakhyaev AV, Tordova M, Gaidarov IO, Popov VI, Fujiwara JH, Chinchilla D, Eisenstein E, Gilliland GL, Ridge KD, Biochemistry. 1998 Oct 6;37(40):13958-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9760230 9760230] | Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure., Abdulaev NG, Karaschuk GN, Ladner JE, Kakuev DL, Yakhyaev AV, Tordova M, Gaidarov IO, Popov VI, Fujiwara JH, Chinchilla D, Eisenstein E, Gilliland GL, Ridge KD, Biochemistry. 1998 Oct 6;37(40):13958-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9760230 9760230] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
| + | [[Category: Nucleoside-diphosphate kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Abdulaev, N.G.]] | [[Category: Abdulaev, N.G.]] | ||
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[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:42:04 2007'' |
Revision as of 11:37, 30 October 2007
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NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA
Overview
The biochemical and structural properties of bovine retinal nucleoside, diphosphate kinase were investigated. The enzyme showed two polypeptides, of approximately 17.5 and 18.5 kDa on SDS-PAGE, while isoelectric focusing, revealed seven to eight proteins with a pI range of 7.4-8.2. Sedimentation, equilibrium yielded a molecular mass of 96 +/- 2 kDa for the enzyme., Carbohydrate analysis revealed that both polypeptides contained Gal, Man, GlcNAc, Fuc, and GalNac saccharides. Like other nucleoside diphosphate, kinases, the retinal enzyme showed substantial differences in the Km, values for various di- and triphosphate nucleotides. Immunogold labeling, of bovine retina revealed that the enzyme is localized on both the, membranes and in the cytoplasm. Screening of a retinal cDNA library, ... [(full description)]
About this Structure
1BE4 is a [Single protein] structure of sequence from [Bos taurus] with PCG as [ligand]. Active as [Nucleoside-diphosphate kinase], with EC number [2.7.4.6]. Structure known Active Site: NUL. Full crystallographic information is available from [OCA].
Reference
Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure., Abdulaev NG, Karaschuk GN, Ladner JE, Kakuev DL, Yakhyaev AV, Tordova M, Gaidarov IO, Popov VI, Fujiwara JH, Chinchilla D, Eisenstein E, Gilliland GL, Ridge KD, Biochemistry. 1998 Oct 6;37(40):13958-67. PMID:9760230
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