1bvk
From Proteopedia
(New page: 200px<br /> <applet load="1bvk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bvk, resolution 2.7Å" /> '''HUMANIZED ANTI-LYSOZ...) |
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| - | [[Image:1bvk.gif|left|200px]]<br /> | + | [[Image:1bvk.gif|left|200px]]<br /><applet load="1bvk" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1bvk" size=" | + | |
caption="1bvk, resolution 2.7Å" /> | caption="1bvk, resolution 2.7Å" /> | ||
'''HUMANIZED ANTI-LYSOZYME FV COMPLEXED WITH LYSOZYME'''<br /> | '''HUMANIZED ANTI-LYSOZYME FV COMPLEXED WITH LYSOZYME'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the complex between hen egg lysozyme and the Fv | + | The crystal structure of the complex between hen egg lysozyme and the Fv fragment of a humanized antilysozyme antibody was determined to 2.7-A resolution. The structure of the antigen combining site in the complex is nearly identical to that of the complexed form of the parent mouse antibody, D1.3. In contrast, the combining sites of the unliganded mouse and humanized antilysozymes show moderate conformational differences. This disparity suggests that a conformational readjustment process linked to antigen binding reverses adverse conformations in the complementarity determining regions that had been introduced by engineering these segments next to human framework regions in the humanized antibody. |
==About this Structure== | ==About this Structure== | ||
| - | 1BVK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1BVK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Buss, T | + | [[Category: Buss, T N.]] |
[[Category: Foote, J.]] | [[Category: Foote, J.]] | ||
| - | [[Category: Holmes, M | + | [[Category: Holmes, M A.]] |
[[Category: anti-lysozyme]] | [[Category: anti-lysozyme]] | ||
[[Category: antibody complex]] | [[Category: antibody complex]] | ||
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[[Category: humanized antibody]] | [[Category: humanized antibody]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:59:34 2008'' |
Revision as of 09:59, 21 February 2008
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HUMANIZED ANTI-LYSOZYME FV COMPLEXED WITH LYSOZYME
Overview
The crystal structure of the complex between hen egg lysozyme and the Fv fragment of a humanized antilysozyme antibody was determined to 2.7-A resolution. The structure of the antigen combining site in the complex is nearly identical to that of the complexed form of the parent mouse antibody, D1.3. In contrast, the combining sites of the unliganded mouse and humanized antilysozymes show moderate conformational differences. This disparity suggests that a conformational readjustment process linked to antigen binding reverses adverse conformations in the complementarity determining regions that had been introduced by engineering these segments next to human framework regions in the humanized antibody.
About this Structure
1BVK is a Protein complex structure of sequences from Gallus gallus and Homo sapiens. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Conformational correction mechanisms aiding antigen recognition by a humanized antibody., Holmes MA, Buss TN, Foote J, J Exp Med. 1998 Feb 16;187(4):479-85. PMID:9463398
Page seeded by OCA on Thu Feb 21 11:59:34 2008
