1by4
From Proteopedia
(New page: 200px<br /> <applet load="1by4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1by4, resolution 2.1Å" /> '''STRUCTURE AND MECHAN...) |
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| - | [[Image:1by4.gif|left|200px]]<br /> | + | [[Image:1by4.gif|left|200px]]<br /><applet load="1by4" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1by4" size=" | + | |
caption="1by4, resolution 2.1Å" /> | caption="1by4, resolution 2.1Å" /> | ||
'''STRUCTURE AND MECHANISM OF THE HOMODIMERIC ASSEMBLY OF THE RXR ON DNA'''<br /> | '''STRUCTURE AND MECHANISM OF THE HOMODIMERIC ASSEMBLY OF THE RXR ON DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 9-cis retinoic acid receptor, RXR, binds DNA effectively as a | + | The 9-cis retinoic acid receptor, RXR, binds DNA effectively as a homodimer or as a heterodimer with other nuclear receptors. The DNA-binding sites for these RXR complexes are direct repeats of a consensus sequence separated by one to five base-pairs of intervening space. Here, we report the 2.1 A crystal structure of the RXR-DNA-binding domain as a homodimer in complex with its idealized direct repeat DNA target. The structure shows how a gene-regulatory site can induce conformational changes in a transcription factor that promote homo-cooperative assembly. Specifically, an alpha-helix in the T-box is disrupted to allow efficient DNA-binding and subunit dimerization. RXR displays a relaxed mode of sequence recognition, interacting with only three base-pairs in each hexameric half-site. The structure illustrates how site selection is achieved in this large eukaryotic transcription factor family through discrete protein-protein interactions and the use of tandem DNA binding sites with characteristic spacings. |
==About this Structure== | ==About this Structure== | ||
| - | 1BY4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1BY4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BY4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ahvazi, B.]] | [[Category: Ahvazi, B.]] | ||
| - | [[Category: Chasse, S | + | [[Category: Chasse, S A.]] |
[[Category: Devarakonda, S.]] | [[Category: Devarakonda, S.]] | ||
[[Category: Rastinejad, F.]] | [[Category: Rastinejad, F.]] | ||
| - | [[Category: Sierk, M | + | [[Category: Sierk, M L.]] |
| - | [[Category: Sigler, P | + | [[Category: Sigler, P B.]] |
[[Category: Zhao, Q.]] | [[Category: Zhao, Q.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: rxr]] | [[Category: rxr]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:00:24 2008'' |
Revision as of 10:00, 21 February 2008
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STRUCTURE AND MECHANISM OF THE HOMODIMERIC ASSEMBLY OF THE RXR ON DNA
Overview
The 9-cis retinoic acid receptor, RXR, binds DNA effectively as a homodimer or as a heterodimer with other nuclear receptors. The DNA-binding sites for these RXR complexes are direct repeats of a consensus sequence separated by one to five base-pairs of intervening space. Here, we report the 2.1 A crystal structure of the RXR-DNA-binding domain as a homodimer in complex with its idealized direct repeat DNA target. The structure shows how a gene-regulatory site can induce conformational changes in a transcription factor that promote homo-cooperative assembly. Specifically, an alpha-helix in the T-box is disrupted to allow efficient DNA-binding and subunit dimerization. RXR displays a relaxed mode of sequence recognition, interacting with only three base-pairs in each hexameric half-site. The structure illustrates how site selection is achieved in this large eukaryotic transcription factor family through discrete protein-protein interactions and the use of tandem DNA binding sites with characteristic spacings.
About this Structure
1BY4 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of RXR-DNA interactions., Zhao Q, Chasse SA, Devarakonda S, Sierk ML, Ahvazi B, Rastinejad F, J Mol Biol. 2000 Feb 18;296(2):509-20. PMID:10669605
Page seeded by OCA on Thu Feb 21 12:00:24 2008
