1c38
From Proteopedia
(New page: 200px<br /><applet load="1c38" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c38" /> '''SOLUTION STRUCTURE OF A QUADRUPLEX FORMING D...) |
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| - | [[Image:1c38.gif|left|200px]]<br /><applet load="1c38" size=" | + | [[Image:1c38.gif|left|200px]]<br /><applet load="1c38" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1c38" /> | caption="1c38" /> | ||
'''SOLUTION STRUCTURE OF A QUADRUPLEX FORMING DNA AND ITS INTERMEDIATE'''<br /> | '''SOLUTION STRUCTURE OF A QUADRUPLEX FORMING DNA AND ITS INTERMEDIATE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Potassium can stabilize the formation of chair- or edge-type quadruplex | + | Potassium can stabilize the formation of chair- or edge-type quadruplex DNA structures and appears to be the only naturally occurring cation that can do so. As quadruplex DNAs may be important in the structure of telomere, centromere, triplet repeat and other DNAs, information about the details of the potassium-quadruplex DNA interactions are of interest. The structures of the 1:1 and the fully saturated, 2:1, potassium-DNA complexes of d(GGTTGGTGTGGTTGG) have been determined using the combination of experimental NMR results and restrained molecular dynamics simulations. The refined structures have been used to model the interactions at the potassium binding sites. Comparison of the 1:1 and 2:1 potassium:DNA structures indicates how potassium binding can determine the folding pattern of the DNA. In each binding site potassium interacts with the carbonyl oxygens of both the loop thymine residues and the guanine residues of the adjacent quartet. |
==About this Structure== | ==About this Structure== | ||
| - | 1C38 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with K as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1C38 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C38 OCA]. |
==Reference== | ==Reference== | ||
Structures of the potassium-saturated, 2:1, and intermediate, 1:1, forms of a quadruplex DNA., Marathias VM, Bolton PH, Nucleic Acids Res. 2000 May 1;28(9):1969-77. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10756199 10756199] | Structures of the potassium-saturated, 2:1, and intermediate, 1:1, forms of a quadruplex DNA., Marathias VM, Bolton PH, Nucleic Acids Res. 2000 May 1;28(9):1969-77. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10756199 10756199] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bolton, P | + | [[Category: Bolton, P H.]] |
| - | [[Category: Marathias, V | + | [[Category: Marathias, V M.]] |
[[Category: Wang, K.]] | [[Category: Wang, K.]] | ||
[[Category: K]] | [[Category: K]] | ||
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[[Category: thrombin binding]] | [[Category: thrombin binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:01:57 2008'' |
Revision as of 10:01, 21 February 2008
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SOLUTION STRUCTURE OF A QUADRUPLEX FORMING DNA AND ITS INTERMEDIATE
Overview
Potassium can stabilize the formation of chair- or edge-type quadruplex DNA structures and appears to be the only naturally occurring cation that can do so. As quadruplex DNAs may be important in the structure of telomere, centromere, triplet repeat and other DNAs, information about the details of the potassium-quadruplex DNA interactions are of interest. The structures of the 1:1 and the fully saturated, 2:1, potassium-DNA complexes of d(GGTTGGTGTGGTTGG) have been determined using the combination of experimental NMR results and restrained molecular dynamics simulations. The refined structures have been used to model the interactions at the potassium binding sites. Comparison of the 1:1 and 2:1 potassium:DNA structures indicates how potassium binding can determine the folding pattern of the DNA. In each binding site potassium interacts with the carbonyl oxygens of both the loop thymine residues and the guanine residues of the adjacent quartet.
About this Structure
1C38 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structures of the potassium-saturated, 2:1, and intermediate, 1:1, forms of a quadruplex DNA., Marathias VM, Bolton PH, Nucleic Acids Res. 2000 May 1;28(9):1969-77. PMID:10756199
Page seeded by OCA on Thu Feb 21 12:01:57 2008
