1c3t
From Proteopedia
(New page: 200px<br /> <applet load="1c3t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c3t" /> '''ROTAMER STRAIN AS A DETERMINANT OF PROTEIN ...) |
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| - | [[Image:1c3t.gif|left|200px]]<br /> | + | [[Image:1c3t.gif|left|200px]]<br /><applet load="1c3t" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''ROTAMER STRAIN AS A DETERMINANT OF PROTEIN STRUCTURAL SPECIFICITY'''<br /> | '''ROTAMER STRAIN AS A DETERMINANT OF PROTEIN STRUCTURAL SPECIFICITY'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We present direct evidence for a change in protein structural specificity | + | We present direct evidence for a change in protein structural specificity due to hydrophobic core packing. High resolution structural analysis of a designed core variant of ubiquitin reveals that the protein is in slow exchange between two conformations. Examination of side-chain rotamers indicates that this dynamic response and the lower stability of the protein are coupled to greater strain and mobility in the core. The results suggest that manipulating the level of side-chain strain may be one way of fine tuning the stability and specificity of proteins. |
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| + | ==Disease== | ||
| + | Known disease associated with this structure: Cleft palate, isolated OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191339 191339]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 1C3T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1C3T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C3T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Desjarlais, J | + | [[Category: Desjarlais, J R.]] |
| - | [[Category: Handel, T | + | [[Category: Handel, T M.]] |
| - | [[Category: Johnson, E | + | [[Category: Johnson, E C.]] |
| - | [[Category: Lazar, G | + | [[Category: Lazar, G A.]] |
[[Category: de novo protein]] | [[Category: de novo protein]] | ||
[[Category: hydrophobic core]] | [[Category: hydrophobic core]] | ||
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[[Category: ubiquitin]] | [[Category: ubiquitin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:02:07 2008'' |
Revision as of 10:02, 21 February 2008
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ROTAMER STRAIN AS A DETERMINANT OF PROTEIN STRUCTURAL SPECIFICITY
Contents |
Overview
We present direct evidence for a change in protein structural specificity due to hydrophobic core packing. High resolution structural analysis of a designed core variant of ubiquitin reveals that the protein is in slow exchange between two conformations. Examination of side-chain rotamers indicates that this dynamic response and the lower stability of the protein are coupled to greater strain and mobility in the core. The results suggest that manipulating the level of side-chain strain may be one way of fine tuning the stability and specificity of proteins.
Disease
Known disease associated with this structure: Cleft palate, isolated OMIM:[191339]
About this Structure
1C3T is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Rotamer strain as a determinant of protein structural specificity., Lazar GA, Johnson EC, Desjarlais JR, Handel TM, Protein Sci. 1999 Dec;8(12):2598-610. PMID:10631975
Page seeded by OCA on Thu Feb 21 12:02:07 2008
