1c3o
From Proteopedia
(New page: 200px<br /><applet load="1c3o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c3o, resolution 2.10Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1c3o.gif|left|200px]]<br /><applet load="1c3o" size=" | + | [[Image:1c3o.gif|left|200px]]<br /><applet load="1c3o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1c3o, resolution 2.10Å" /> | caption="1c3o, resolution 2.10Å" /> | ||
'''CRYSTAL STRUCTURE OF THE CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT MUTANT C269S WITH BOUND GLUTAMINE'''<br /> | '''CRYSTAL STRUCTURE OF THE CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT MUTANT C269S WITH BOUND GLUTAMINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Carbamoyl phosphate synthetase (CPS) plays a key role in both arginine and | + | Carbamoyl phosphate synthetase (CPS) plays a key role in both arginine and pyrimidine biosynthesis by catalyzing the production of carbamoyl phosphate. The enzyme from Escherichi coli consists of two polypeptide chains referred to as the small and large subunits. On the basis of both amino acid sequence analyses and X-ray structural studies, it is known that the small subunit belongs to the Triad or Type I class of amidotransferases, all of which contain a cysteine-histidine (Cys269 and His353) couple required for activity. The hydrolysis of glutamine by the small subunit has been proposed to occur via two tetrahedral intermediates and a glutamyl-thioester moiety. Here, we describe the three-dimensional structures of the C269S/glutamine and CPS/glutamate gamma-semialdehyde complexes, which serve as mimics for the Michaelis complex and the tetrahedral intermediates, respectively. In conjunction with the previously solved glutamyl-thioester intermediate complex, the stereochemical course of glutamine hydrolysis in CPS has been outlined. Specifically, attack by the thiolate of Cys269 occurs at the Si face of the carboxamide group of the glutamine substrate leading to a tetrahedral intermediate with an S-configuration. Both the backbone amide groups of Gly241 and Leu270, and O(gamma) of Ser47 play key roles in stabilizing the developing oxyanion. Collapse of the tetrahedral intermediate leads to formation of the glutamyl-thioester intermediate, which is subsequently attacked at the Si face by an activated water molecule positioned near His353. The results described here serve as a paradigm for other members of the Triad class of amidotranferases. |
==About this Structure== | ==About this Structure== | ||
| - | 1C3O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MN, K, CL, PO4, ORN, GLN, NET and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ligase Ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.5.5. 6.3.5.5.] Full crystallographic information is available from [http:// | + | 1C3O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=ORN:'>ORN</scene>, <scene name='pdbligand=GLN:'>GLN</scene>, <scene name='pdbligand=NET:'>NET</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ligase Ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.5.5. 6.3.5.5.] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C3O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ligase]] | [[Category: Ligase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Holden, H | + | [[Category: Holden, H M.]] |
[[Category: Huang, X.]] | [[Category: Huang, X.]] | ||
| - | [[Category: Raushel, F | + | [[Category: Raushel, F M.]] |
| - | [[Category: Thoden, J | + | [[Category: Thoden, J B.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: michaelis complex]] | [[Category: michaelis complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:02:09 2008'' |
Revision as of 10:02, 21 February 2008
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CRYSTAL STRUCTURE OF THE CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT MUTANT C269S WITH BOUND GLUTAMINE
Overview
Carbamoyl phosphate synthetase (CPS) plays a key role in both arginine and pyrimidine biosynthesis by catalyzing the production of carbamoyl phosphate. The enzyme from Escherichi coli consists of two polypeptide chains referred to as the small and large subunits. On the basis of both amino acid sequence analyses and X-ray structural studies, it is known that the small subunit belongs to the Triad or Type I class of amidotransferases, all of which contain a cysteine-histidine (Cys269 and His353) couple required for activity. The hydrolysis of glutamine by the small subunit has been proposed to occur via two tetrahedral intermediates and a glutamyl-thioester moiety. Here, we describe the three-dimensional structures of the C269S/glutamine and CPS/glutamate gamma-semialdehyde complexes, which serve as mimics for the Michaelis complex and the tetrahedral intermediates, respectively. In conjunction with the previously solved glutamyl-thioester intermediate complex, the stereochemical course of glutamine hydrolysis in CPS has been outlined. Specifically, attack by the thiolate of Cys269 occurs at the Si face of the carboxamide group of the glutamine substrate leading to a tetrahedral intermediate with an S-configuration. Both the backbone amide groups of Gly241 and Leu270, and O(gamma) of Ser47 play key roles in stabilizing the developing oxyanion. Collapse of the tetrahedral intermediate leads to formation of the glutamyl-thioester intermediate, which is subsequently attacked at the Si face by an activated water molecule positioned near His353. The results described here serve as a paradigm for other members of the Triad class of amidotranferases.
About this Structure
1C3O is a Protein complex structure of sequences from [1] with , , , , , , and as ligands. Active as Ligase, with EC number 6.3.5.5. Full crystallographic information is available from OCA.
Reference
The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway., Thoden JB, Huang X, Raushel FM, Holden HM, Biochemistry. 1999 Dec 7;38(49):16158-66. PMID:10587438 [[Category: ]]
Page seeded by OCA on Thu Feb 21 12:02:09 2008
Categories: Ligase | Protein complex | Holden, H M. | Huang, X. | Raushel, F M. | Thoden, J B. | ADP | CL | GLN | K | MN | NET | ORN | PO4 | Amidotransferase | Atp-grasp | Michaelis complex
