1c57
From Proteopedia
(New page: 200px<br /><applet load="1c57" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c57, resolution 2.4Å" /> '''DIRECT DETERMINATION ...) |
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| - | [[Image:1c57.gif|left|200px]]<br /><applet load="1c57" size=" | + | [[Image:1c57.gif|left|200px]]<br /><applet load="1c57" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1c57, resolution 2.4Å" /> | caption="1c57, resolution 2.4Å" /> | ||
'''DIRECT DETERMINATION OF THE POSITIONS OF DEUTERIUM ATOMS OF BOUND WATER IN CONCANAVALIN A BY NEUTRON LAUE CRYSTALLOGRAPHY'''<br /> | '''DIRECT DETERMINATION OF THE POSITIONS OF DEUTERIUM ATOMS OF BOUND WATER IN CONCANAVALIN A BY NEUTRON LAUE CRYSTALLOGRAPHY'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The correct positions of the deuterium (D) atoms of many of the bound | + | The correct positions of the deuterium (D) atoms of many of the bound waters in the protein concanavalin A are revealed by neutron Laue diffraction. The approach includes cases where these water D atoms show enough mobility to render them invisible even to ultra-high resolution synchrotron-radiation X-ray crystallography. The positions of the bound water H atoms calculated on the basis of chemical and energetic considerations are often incorrect. The D-atom positions for the water molecules in the Mn-, Ca- and sugar-binding sites of concanavalin A are described in detail. |
==About this Structure== | ==About this Structure== | ||
| - | 1C57 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with MN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1C57 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C57 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Canavalia ensiformis]] | [[Category: Canavalia ensiformis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Gilboa, A | + | [[Category: Gilboa, A J.Kalb.]] |
[[Category: Habash, J.]] | [[Category: Habash, J.]] | ||
| - | [[Category: Helliwell, J | + | [[Category: Helliwell, J R.]] |
| - | [[Category: Lehmann, M | + | [[Category: Lehmann, M S.]] |
[[Category: Nuttall, R.]] | [[Category: Nuttall, R.]] | ||
| - | [[Category: Price, H | + | [[Category: Price, H J.]] |
[[Category: Raftery, J.]] | [[Category: Raftery, J.]] | ||
[[Category: Wilkinson, C.]] | [[Category: Wilkinson, C.]] | ||
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[[Category: neutron laue diffraction]] | [[Category: neutron laue diffraction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:02:30 2008'' |
Revision as of 10:02, 21 February 2008
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DIRECT DETERMINATION OF THE POSITIONS OF DEUTERIUM ATOMS OF BOUND WATER IN CONCANAVALIN A BY NEUTRON LAUE CRYSTALLOGRAPHY
Overview
The correct positions of the deuterium (D) atoms of many of the bound waters in the protein concanavalin A are revealed by neutron Laue diffraction. The approach includes cases where these water D atoms show enough mobility to render them invisible even to ultra-high resolution synchrotron-radiation X-ray crystallography. The positions of the bound water H atoms calculated on the basis of chemical and energetic considerations are often incorrect. The D-atom positions for the water molecules in the Mn-, Ca- and sugar-binding sites of concanavalin A are described in detail.
About this Structure
1C57 is a Single protein structure of sequence from Canavalia ensiformis with and as ligands. Full crystallographic information is available from OCA.
Reference
Direct determination of the positions of the deuterium atoms of the bound water in -concanavalin A by neutron Laue crystallography., Habash J, Raftery J, Nuttall R, Price HJ, Wilkinson C, Kalb AJ, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):541-50. PMID:10771422
Page seeded by OCA on Thu Feb 21 12:02:30 2008
