2cjl
From Proteopedia
(New page: 200px<br /> <applet load="2cjl" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cjl, resolution 1.50Å" /> '''CRYSTAL STRUCTURE A...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2CJL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CJL OCA]]. | + | 2CJL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Chitinase Chitinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CJL OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes., Hoell IA, Dalhus B, Heggset EB, Aspmo SI, Eijsink VG, FEBS J. 2006 Nov;273(21):4889-900. Epub 2006 Sep 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17010167 17010167] | Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes., Hoell IA, Dalhus B, Heggset EB, Aspmo SI, Eijsink VG, FEBS J. 2006 Nov;273(21):4889-900. Epub 2006 Sep 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17010167 17010167] | ||
| + | [[Category: Chitinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces coelicolor]] | [[Category: Streptomyces coelicolor]] | ||
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[[Category: plant enzymes]] | [[Category: plant enzymes]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:43:37 2007'' |
Revision as of 11:38, 30 October 2007
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CRYSTAL STRUCTURE AND ENZYMATIC PROPERTIES OF A BACTERIAL FAMILY 19 CHITINASE REVEAL DIFFERENCES WITH PLANT ENZYMES
Overview
We describe the cloning, overexpression, purification, characterization, and crystal structure of chitinase G, a single-domain family 19 chitinase, from the Gram-positive bacterium Streptomyces coelicolor A3(2). Although, chitinase G was not capable of releasing 4-methylumbelliferyl from, artificial chitooligosaccharide substrates, it was capable of degrading, longer chitooligosaccharides at rates similar to those observed for other, chitinases. The enzyme was also capable of degrading a colored colloidal, chitin substrate (carboxymethyl-chitin-remazol-brilliant violet) and a, small, presumably amorphous, subfraction of alpha-chitin and beta-chitin, but was not capable of degrading crystalline chitin completely. The, crystal structures of chitinase G and a related Streptomyces chitinase, ... [(full description)]
About this Structure
2CJL is a [Single protein] structure of sequence from [Streptomyces coelicolor] with ZN as [ligand]. Active as [Chitinase], with EC number [3.2.1.14]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes., Hoell IA, Dalhus B, Heggset EB, Aspmo SI, Eijsink VG, FEBS J. 2006 Nov;273(21):4889-900. Epub 2006 Sep 28. PMID:17010167
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