1c8o
From Proteopedia
(New page: 200px<br /><applet load="1c8o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c8o, resolution 2.90Å" /> '''2.9 A STRUCTURE OF C...) |
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| - | [[Image:1c8o.gif|left|200px]]<br /><applet load="1c8o" size=" | + | [[Image:1c8o.gif|left|200px]]<br /><applet load="1c8o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1c8o, resolution 2.90Å" /> | caption="1c8o, resolution 2.90Å" /> | ||
'''2.9 A STRUCTURE OF CLEAVED VIRAL SERPIN CRMA'''<br /> | '''2.9 A STRUCTURE OF CLEAVED VIRAL SERPIN CRMA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | CrmA is an unusual viral serpin that inhibits both cysteine and serine | + | CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS-stable covalent complexes with cysteine proteinases. To obtain insight into the inhibitory mechanism of crmA, we determined the crystal structure of reactive center loop-cleaved crmA to 2.9 A resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for b sheet A, whose sequence and length are unique to crmA. |
==About this Structure== | ==About this Structure== | ||
| - | 1C8O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Cupixi_virus Cupixi virus]. Full crystallographic information is available from [http:// | + | 1C8O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Cupixi_virus Cupixi virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cupixi virus]] | [[Category: Cupixi virus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Gettins, P | + | [[Category: Gettins, P G.W.]] |
[[Category: Simonovic, M.]] | [[Category: Simonovic, M.]] | ||
[[Category: Volz, K.]] | [[Category: Volz, K.]] | ||
[[Category: serpin fold]] | [[Category: serpin fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:03:34 2008'' |
Revision as of 10:03, 21 February 2008
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2.9 A STRUCTURE OF CLEAVED VIRAL SERPIN CRMA
Overview
CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS-stable covalent complexes with cysteine proteinases. To obtain insight into the inhibitory mechanism of crmA, we determined the crystal structure of reactive center loop-cleaved crmA to 2.9 A resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for b sheet A, whose sequence and length are unique to crmA.
About this Structure
1C8O is a Protein complex structure of sequences from Cupixi virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition., Simonovic M, Gettins PGW, Volz K, Protein Sci. 2000 Aug;9(8):1423-7. PMID:10975564
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