1cd8
From Proteopedia
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==Overview== | ==Overview== | ||
| - | A secreted fragment of the extracellular portion of human CD8 alpha has | + | A secreted fragment of the extracellular portion of human CD8 alpha has been expressed in CHO cells, and a deglycosylated and proteolyzed form of this fragment has been crystallized. We report here the crystal structure of this fragment as refined at 2.6 A resolution. The structure was solved by molecular replacement using a superposition of ten variable domains from immunoglobulin light chains as the search model. Only the N-terminal 114 amino acids of CD8 alpha are visible in the electron density maps. The domain formed by these residues possesses a fold typical of immunoglobulin variable domains and associates to form Fv-like homodimers. |
==Disease== | ==Disease== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Axel, R.]] | [[Category: Axel, R.]] | ||
| - | [[Category: Hendrickson, W | + | [[Category: Hendrickson, W A.]] |
| - | [[Category: Leahy, D | + | [[Category: Leahy, D J.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: surface glycoprotein]] | [[Category: surface glycoprotein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:04:50 2008'' |
Revision as of 10:04, 21 February 2008
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CRYSTAL STRUCTURE OF A SOLUBLE FORM OF THE HUMAN T CELL CO-RECEPTOR CD8 AT 2.6 ANGSTROMS RESOLUTION
Contents |
Overview
A secreted fragment of the extracellular portion of human CD8 alpha has been expressed in CHO cells, and a deglycosylated and proteolyzed form of this fragment has been crystallized. We report here the crystal structure of this fragment as refined at 2.6 A resolution. The structure was solved by molecular replacement using a superposition of ten variable domains from immunoglobulin light chains as the search model. Only the N-terminal 114 amino acids of CD8 alpha are visible in the electron density maps. The domain formed by these residues possesses a fold typical of immunoglobulin variable domains and associates to form Fv-like homodimers.
Disease
Known diseases associated with this structure: CD8 deficiency, familial OMIM:[186910], Megakaryoblastic leukemia, acute OMIM:[606078]
About this Structure
1CD8 is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution., Leahy DJ, Axel R, Hendrickson WA, Cell. 1992 Mar 20;68(6):1145-62. PMID:1547508
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