1cfm
From Proteopedia
(New page: 200px<br /><applet load="1cfm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cfm, resolution 2.00Å" /> '''CYTOCHROME F FROM CH...) |
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| - | [[Image:1cfm.jpg|left|200px]]<br /><applet load="1cfm" size=" | + | [[Image:1cfm.jpg|left|200px]]<br /><applet load="1cfm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cfm, resolution 2.00Å" /> | caption="1cfm, resolution 2.00Å" /> | ||
'''CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII'''<br /> | '''CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A truncated form of cytochrome f from Chlamydomonas reinhardtii (an | + | A truncated form of cytochrome f from Chlamydomonas reinhardtii (an important eukaryotic model organism for photosynthetic electron transfer studies) has been crystallized (space group P2(1)2(1)2(1); three molecules/asymmetric unit) and its structure determined to 2.0 A resolution by molecular replacement using the coordinates of a truncated turnip cytochrome f as a model. The structure displays the same folding and detailed features as turnip cytochrome f, including (a) an unusual heme Fe ligation by the alpha-amino group of tyrosine 1, (b) a cluster of lysine residues (proposed docking site of plastocyanin), and (c) the presence of a chain of seven water molecules bound to conserved residues and extending between the heme pocket and K58 and K66 at the lysine cluster. For this array of waters, we propose a structural role. Two cytochrome f molecules are related by a noncrystallographic symmetry operator which is a distorted proper 2-fold rotation. This may represent the dimeric relation of the monomers in situ; however, the heme orientation suggested by this model is not consistent with previous EPR measurements on oriented membranes. |
==About this Structure== | ==About this Structure== | ||
| - | 1CFM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1CFM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berry, E | + | [[Category: Berry, E A.]] |
| - | [[Category: Chi, Y | + | [[Category: Chi, Y I.]] |
| - | [[Category: Fernandez-Velasco, J | + | [[Category: Fernandez-Velasco, J G.]] |
| - | [[Category: Huang, L | + | [[Category: Huang, L S.]] |
[[Category: Malkin, R.]] | [[Category: Malkin, R.]] | ||
[[Category: Zhang, Z.]] | [[Category: Zhang, Z.]] | ||
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[[Category: proton wire]] | [[Category: proton wire]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:34 2008'' |
Revision as of 10:05, 21 February 2008
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CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII
Overview
A truncated form of cytochrome f from Chlamydomonas reinhardtii (an important eukaryotic model organism for photosynthetic electron transfer studies) has been crystallized (space group P2(1)2(1)2(1); three molecules/asymmetric unit) and its structure determined to 2.0 A resolution by molecular replacement using the coordinates of a truncated turnip cytochrome f as a model. The structure displays the same folding and detailed features as turnip cytochrome f, including (a) an unusual heme Fe ligation by the alpha-amino group of tyrosine 1, (b) a cluster of lysine residues (proposed docking site of plastocyanin), and (c) the presence of a chain of seven water molecules bound to conserved residues and extending between the heme pocket and K58 and K66 at the lysine cluster. For this array of waters, we propose a structural role. Two cytochrome f molecules are related by a noncrystallographic symmetry operator which is a distorted proper 2-fold rotation. This may represent the dimeric relation of the monomers in situ; however, the heme orientation suggested by this model is not consistent with previous EPR measurements on oriented membranes.
About this Structure
1CFM is a Single protein structure of sequence from Chlamydomonas reinhardtii with as ligand. Full crystallographic information is available from OCA.
Reference
X-ray structure of a truncated form of cytochrome f from chlamydomonas reinhardtii., Chi YI, Huang LS, Zhang Z, Fernandez-Velasco JG, Berry EA, Biochemistry. 2000 Jul 4;39(26):7689-701. PMID:10869174
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