1cfz
From Proteopedia
(New page: 200px<br /><applet load="1cfz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cfz, resolution 2.200Å" /> '''HYDROGENASE MATURAT...) |
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| - | [[Image:1cfz.jpg|left|200px]]<br /><applet load="1cfz" size=" | + | [[Image:1cfz.jpg|left|200px]]<br /><applet load="1cfz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cfz, resolution 2.200Å" /> | caption="1cfz, resolution 2.200Å" /> | ||
'''HYDROGENASE MATURATING ENDOPEPTIDASE HYBD FROM E. COLI'''<br /> | '''HYDROGENASE MATURATING ENDOPEPTIDASE HYBD FROM E. COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The maturation of [NiFe] hydrogenases includes formation of the nickel | + | The maturation of [NiFe] hydrogenases includes formation of the nickel metallocenter, proteolytic processing of the metal center carrying large subunit, and its assembling with other hydrogenase subunits. The hydrogenase maturating enzyme HYBD from Escherichia coli, a protease of molecular mass 17.5 kDa, specifically cleaves off a 15 amino acid peptide from the C terminus of the precursor of the large subunit of hydrogenase 2 in a nickel-dependent manner. Here we report the crystal structure of HYBD at 2.2 A resolution. It consists of a twisted five-stranded beta-sheet surrounded by four and three helices, respectively, on each side. A cadmium ion from the crystallization buffer binds to the proposed nickel-binding site and is penta-coordinated by Glu16, Asp62, His93, and a water molecule in a pseudo-tetragonal arrangement. HYBD is topologically related to members of the metzincins superfamily of zinc endoproteinases, sharing the central beta-sheet and three helices. In contrast to the metzincins, the metal-binding site of HYBD is localized at the C-terminal end of the beta-sheet. Three helical insertions unique to HYBD pack against one side of the sheet, build up the active site cleft, and provide His93 as ligand to the metal. From this structure, we derive molecular clues into how the protease HYBD is involved in the hydrogenase maturation process. |
==About this Structure== | ==About this Structure== | ||
| - | 1CFZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1CFZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Beisel, H | + | [[Category: Beisel, H G.]] |
[[Category: Boeck, A.]] | [[Category: Boeck, A.]] | ||
[[Category: Fritsche, E.]] | [[Category: Fritsche, E.]] | ||
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[[Category: protease]] | [[Category: protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:40 2008'' |
Revision as of 10:05, 21 February 2008
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HYDROGENASE MATURATING ENDOPEPTIDASE HYBD FROM E. COLI
Overview
The maturation of [NiFe] hydrogenases includes formation of the nickel metallocenter, proteolytic processing of the metal center carrying large subunit, and its assembling with other hydrogenase subunits. The hydrogenase maturating enzyme HYBD from Escherichia coli, a protease of molecular mass 17.5 kDa, specifically cleaves off a 15 amino acid peptide from the C terminus of the precursor of the large subunit of hydrogenase 2 in a nickel-dependent manner. Here we report the crystal structure of HYBD at 2.2 A resolution. It consists of a twisted five-stranded beta-sheet surrounded by four and three helices, respectively, on each side. A cadmium ion from the crystallization buffer binds to the proposed nickel-binding site and is penta-coordinated by Glu16, Asp62, His93, and a water molecule in a pseudo-tetragonal arrangement. HYBD is topologically related to members of the metzincins superfamily of zinc endoproteinases, sharing the central beta-sheet and three helices. In contrast to the metzincins, the metal-binding site of HYBD is localized at the C-terminal end of the beta-sheet. Three helical insertions unique to HYBD pack against one side of the sheet, build up the active site cleft, and provide His93 as ligand to the metal. From this structure, we derive molecular clues into how the protease HYBD is involved in the hydrogenase maturation process.
About this Structure
1CFZ is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the hydrogenase maturating endopeptidase HYBD from Escherichia coli., Fritsche E, Paschos A, Beisel HG, Bock A, Huber R, J Mol Biol. 1999 May 21;288(5):989-98. PMID:10331925
Page seeded by OCA on Thu Feb 21 12:05:40 2008
Categories: Escherichia coli | Single protein | Beisel, H G. | Boeck, A. | Fritsche, E. | Huber, R. | Paschos, A. | CD | Hydrogenase | Maturation | Metzincins | Nickel | Protease
