4rsd
From Proteopedia
(New page: 200px<br /> <applet load="4rsd" size="450" color="white" frame="true" align="right" spinBox="true" caption="4rsd, resolution 1.6Å" /> '''STRUCTURE OF THE D12...) |
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==About this Structure== | ==About this Structure== | ||
| - | 4RSD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with ACT and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4RSD OCA]]. | + | 4RSD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with ACT and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]]. Structure known Active Sites: B1, B2, P1 and P2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4RSD OCA]]. |
==Reference== | ==Reference== | ||
His...Asp catalytic dyad of ribonuclease A: structure and function of the wild-type, D121N, and D121A enzymes., Schultz LW, Quirk DJ, Raines RT, Biochemistry. 1998 Jun 23;37(25):8886-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9636030 9636030] | His...Asp catalytic dyad of ribonuclease A: structure and function of the wild-type, D121N, and D121A enzymes., Schultz LW, Quirk DJ, Raines RT, Biochemistry. 1998 Jun 23;37(25):8886-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9636030 9636030] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
| + | [[Category: Pancreatic ribonuclease]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Quirk, D.J.]] | [[Category: Quirk, D.J.]] | ||
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[[Category: x-ray diffraction]] | [[Category: x-ray diffraction]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:45:11 2007'' |
Revision as of 11:40, 30 October 2007
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STRUCTURE OF THE D121A VARIANT OF RIBONUCLEASE A
Overview
The side chains of histidine and aspartate residues form a hydrogen bond, in the active sites of many enzymes. In serine proteases, the His...Asp, hydrogen bond of the catalytic triad is known to contribute greatly to, catalysis, perhaps via the formation of a low-barrier hydrogen bond. In, bovine pancreatic ribonuclease A (RNase A), the His...Asp dyad is composed, of His119 and Asp121. Previously, site-directed mutagenesis was used to, show that His119 has a fundamental role, to act as an acid during, catalysis of RNA cleavage [Thompson, J. E., and Raines, R. T. (1994) J., Am. Chem. Soc. 116, 5467-5468]. Here, Asp121 was replaced with an, asparagine or alanine residue. The crystalline structures of the two, variants were determined by X-ray diffraction analysis to a resolution of, 1.6 A ... [(full description)]
About this Structure
4RSD is a [Single protein] structure of sequence from [Bos taurus] with ACT and CL as [ligands]. Active as [Pancreatic ribonuclease], with EC number [3.1.27.5]. Structure known Active Sites: B1, B2, P1 and P2. Full crystallographic information is available from [OCA].
Reference
His...Asp catalytic dyad of ribonuclease A: structure and function of the wild-type, D121N, and D121A enzymes., Schultz LW, Quirk DJ, Raines RT, Biochemistry. 1998 Jun 23;37(25):8886-98. PMID:9636030
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