1cj5
From Proteopedia
(New page: 200px<br /><applet load="1cj5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cj5" /> '''BOVINE BETA-LACTOGLOBULIN A'''<br /> ==Over...) |
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| - | [[Image:1cj5.gif|left|200px]]<br /><applet load="1cj5" size=" | + | [[Image:1cj5.gif|left|200px]]<br /><applet load="1cj5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cj5" /> | caption="1cj5" /> | ||
'''BOVINE BETA-LACTOGLOBULIN A'''<br /> | '''BOVINE BETA-LACTOGLOBULIN A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Using heteronuclear NMR spectroscopy, we studied the solution structure | + | Using heteronuclear NMR spectroscopy, we studied the solution structure and dynamics of bovine beta-lactoglobulin A at pH 2.0 and 45 degrees C, where the protein exists as a monomeric native state. The monomeric NMR structure, comprising an eight-stranded continuous antiparallel beta-barrel and one major alpha-helix, is similar to the X-ray dimeric structure obtained at pH 6.2, including betaI-strand that forms the dimer interface and loop EF that serves as a lid of the interior hydrophobic hole. [1H]-15N NOE revealed that betaF, betaG, and betaH strands buried under the major alpha-helix are rigid on a pico- to nanosecond time scale and also emphasized rapid fluctuations of loops and the N- and C-terminal regions. |
==About this Structure== | ==About this Structure== | ||
| - | 1CJ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http:// | + | 1CJ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CJ5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Batt, C | + | [[Category: Batt, C A.]] |
[[Category: Era, S.]] | [[Category: Era, S.]] | ||
[[Category: Forge, V.]] | [[Category: Forge, V.]] | ||
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[[Category: dynamics]] | [[Category: dynamics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:06:41 2008'' |
Revision as of 10:06, 21 February 2008
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BOVINE BETA-LACTOGLOBULIN A
Overview
Using heteronuclear NMR spectroscopy, we studied the solution structure and dynamics of bovine beta-lactoglobulin A at pH 2.0 and 45 degrees C, where the protein exists as a monomeric native state. The monomeric NMR structure, comprising an eight-stranded continuous antiparallel beta-barrel and one major alpha-helix, is similar to the X-ray dimeric structure obtained at pH 6.2, including betaI-strand that forms the dimer interface and loop EF that serves as a lid of the interior hydrophobic hole. [1H]-15N NOE revealed that betaF, betaG, and betaH strands buried under the major alpha-helix are rigid on a pico- to nanosecond time scale and also emphasized rapid fluctuations of loops and the N- and C-terminal regions.
About this Structure
1CJ5 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Solution structure and dynamics of bovine beta-lactoglobulin A., Kuwata K, Hoshino M, Forge V, Era S, Batt CA, Goto Y, Protein Sci. 1999 Nov;8(11):2541-5. PMID:10595563
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