2j4e
From Proteopedia
(New page: 200px<br /> <applet load="2j4e" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j4e, resolution 2.80Å" /> '''THE ITP COMPLEX OF ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2J4E is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG, ITT, POP and IMP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.19 3.6.1.19]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J4E OCA]]. | + | 2J4E is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG, ITT, POP and IMP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Nucleoside-triphosphate_diphosphatase Nucleoside-triphosphate diphosphatase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.19 3.6.1.19]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J4E OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of human inosine triphosphatase. Substrate binding and implication of the inosine triphosphatase deficiency mutation P32T., Stenmark P, Kursula P, Flodin S, Graslund S, Landry R, Nordlund P, Schuler H, J Biol Chem. 2007 Feb 2;282(5):3182-7. Epub 2006 Nov 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17138556 17138556] | Crystal structure of human inosine triphosphatase. Substrate binding and implication of the inosine triphosphatase deficiency mutation P32T., Stenmark P, Kursula P, Flodin S, Graslund S, Landry R, Nordlund P, Schuler H, J Biol Chem. 2007 Feb 2;282(5):3182-7. Epub 2006 Nov 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17138556 17138556] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| + | [[Category: Nucleoside-triphosphate diphosphatase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arrowsmith, C.]] | [[Category: Arrowsmith, C.]] | ||
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[[Category: nucleotide metabolism]] | [[Category: nucleotide metabolism]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:45:43 2007'' |
Revision as of 11:41, 30 October 2007
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THE ITP COMPLEX OF HUMAN INOSINE TRIPHOSPHATASE
Overview
Inosine triphosphatase (ITPA) is a ubiquitous key regulator of cellular, non-canonical nucleotide levels. It breaks down inosine and xanthine, nucleotides generated by deamination of purine bases. Its enzymatic action, prevents accumulation of ITP and reduces the risk of incorporation of, potentially mutagenic inosine nucleotides into nucleic acids. Here we, describe the crystal structure of human ITPA in complex with its prime, substrate ITP, as well as the apoenzyme at 2.8 and 1.1A, respectively., These structures show for the first time the site of substrate and Mg2+, coordination as well as the conformational changes accompanying substrate, binding in this class of enzymes. Enzyme substrate interactions induce an, extensive closure of the nucleotide binding grove, resulting in tight, ... [(full description)]
About this Structure
2J4E is a [Single protein] structure of sequence from [Homo sapiens] with MG, ITT, POP and IMP as [ligands]. Active as [Nucleoside-triphosphate diphosphatase], with EC number [3.6.1.19]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of human inosine triphosphatase. Substrate binding and implication of the inosine triphosphatase deficiency mutation P32T., Stenmark P, Kursula P, Flodin S, Graslund S, Landry R, Nordlund P, Schuler H, J Biol Chem. 2007 Feb 2;282(5):3182-7. Epub 2006 Nov 29. PMID:17138556
Page seeded by OCA on Tue Oct 30 13:45:43 2007
Categories: Homo sapiens | Nucleoside-triphosphate diphosphatase | Single protein | Arrowsmith, C. | Berg, S.Van.Den. | Berglund, H. | Busam, R. | Collins, R. | Edwards, A. | Ehn, M. | Flodin, S. | Flores, A. | Graslund, S. | Hallberg, B.M. | Hammarstrom, M. | Hogbom, M. | Kotenyova, T. | Kursula, P. | Landry, R. | Loppnau, P. | Magnusdottir, A. | Nilsson-Ehle, P. | Nordlund, P. | Nyman, T. | Ogg, D. | Persson, C. | Sagemark, J. | Schiavone, L.Holmberg. | Schuler, H. | Stenmark, P. | Sundstrom, M. | Thorsell, A.G. | Uppenberg, J. | Wallden, K. | Weigelt, J. | IMP | ITT | MG | POP | Disease mutation | Hydrolase | Imp | Inosine triphosphatase deficiency | Inosine triphosphate pyrophosphohydrolase | Itp | Nucleotide metabolism
