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2j4g
From Proteopedia
(New page: 200px<br /> <applet load="2j4g" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j4g, resolution 2.25Å" /> '''BACTEROIDES THETAIO...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2J4G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]] with ACT, NB1 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J4G OCA]]. | + | 2J4G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]] with ACT, NB1 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J4G OCA]]. |
==Reference== | ==Reference== | ||
Analysis of PUGNAc and NAG-thiazoline as transition state analogues for human O-GlcNAcase: mechanistic and structural insights into inhibitor selectivity and transition state poise., Whitworth GE, Macauley MS, Stubbs KA, Dennis RJ, Taylor EJ, Davies GJ, Greig IR, Vocadlo DJ, J Am Chem Soc. 2007 Jan 24;129(3):635-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17227027 17227027] | Analysis of PUGNAc and NAG-thiazoline as transition state analogues for human O-GlcNAcase: mechanistic and structural insights into inhibitor selectivity and transition state poise., Whitworth GE, Macauley MS, Stubbs KA, Dennis RJ, Taylor EJ, Davies GJ, Greig IR, Vocadlo DJ, J Am Chem Soc. 2007 Jan 24;129(3):635-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17227027 17227027] | ||
[[Category: Bacteroides thetaiotaomicron]] | [[Category: Bacteroides thetaiotaomicron]] | ||
| + | [[Category: Beta-N-acetylhexosaminidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Davies, G.J.]] | [[Category: Davies, G.J.]] | ||
| Line 26: | Line 27: | ||
[[Category: thiazoline]] | [[Category: thiazoline]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:45:54 2007'' |
Revision as of 11:41, 30 October 2007
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BACTEROIDES THETAIOTAOMICRON GH84 O-GLCNACASE IN COMPLEX WITH N-BUTYL-THIAZOLINE INHIBITOR
Overview
O-GlcNAcase catalyzes the cleavage of beta-O-linked, 2-acetamido-2-deoxy-beta-d-glucopyranoside (O-GlcNAc) from serine and, threonine residues of post-translationally modified proteins. Two potent, inhibitors of this enzyme are, O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino-N-phenylcarbamate, (PUGNAc) and, 1,2-dideoxy-2'-methyl-alpha-d-glucopyranoso[2,1-d]-Delta2'-thiazoline, (NAG-thiazoline). Derivatives of these inhibitors differ in their, selectivity for human O-GlcNAcase over the functionally related human, lysosomal beta-hexosamindases, with PUGNAc derivatives showing modest, selectivities and NAG-thiazoline derivatives showing high selectivities., The molecular basis for this difference in selectivities is addressed as, is how well these inhibitors mimic the ... [(full description)]
About this Structure
2J4G is a [Single protein] structure of sequence from [Bacteroides thetaiotaomicron] with ACT, NB1 and GOL as [ligands]. Active as [Beta-N-acetylhexosaminidase], with EC number [3.2.1.52]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Analysis of PUGNAc and NAG-thiazoline as transition state analogues for human O-GlcNAcase: mechanistic and structural insights into inhibitor selectivity and transition state poise., Whitworth GE, Macauley MS, Stubbs KA, Dennis RJ, Taylor EJ, Davies GJ, Greig IR, Vocadlo DJ, J Am Chem Soc. 2007 Jan 24;129(3):635-44. PMID:17227027
Page seeded by OCA on Tue Oct 30 13:45:54 2007
Categories: Bacteroides thetaiotaomicron | Beta-N-acetylhexosaminidase | Single protein | Davies, G.J. | Dennis, R.J. | ACT | GOL | NB1 | Enzyme | Gh84 | Inhibition | Inhibitor | O-glcnacase | Thiazoline
