1cld
From Proteopedia
(New page: 200px<br /><applet load="1cld" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cld" /> '''DNA-BINDING PROTEIN'''<br /> ==Overview== T...) |
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| - | [[Image:1cld.jpg|left|200px]]<br /><applet load="1cld" size=" | + | [[Image:1cld.jpg|left|200px]]<br /><applet load="1cld" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cld" /> | caption="1cld" /> | ||
'''DNA-BINDING PROTEIN'''<br /> | '''DNA-BINDING PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Zn2Cys6 DNA-binding domain has been identified by sequence homology in | + | The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1CLD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1CLD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kluyveromyces lactis]] | [[Category: Kluyveromyces lactis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Coleman, J | + | [[Category: Coleman, J E.]] |
| - | [[Category: Gardner, K | + | [[Category: Gardner, K H.]] |
[[Category: CD]] | [[Category: CD]] | ||
[[Category: zinc-binding domain]] | [[Category: zinc-binding domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:16 2008'' |
Revision as of 10:07, 21 February 2008
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DNA-BINDING PROTEIN
Overview
The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.
About this Structure
1CLD is a Single protein structure of sequence from Kluyveromyces lactis with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain., Gardner KH, Anderson SF, Coleman JE, Nat Struct Biol. 1995 Oct;2(10):898-905. PMID:7552715
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