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1cma
From Proteopedia
(New page: 200px<br /><applet load="1cma" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cma, resolution 2.800Å" /> '''MET REPRESSOR/DNA C...) |
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| - | [[Image:1cma.jpg|left|200px]]<br /><applet load="1cma" size=" | + | [[Image:1cma.jpg|left|200px]]<br /><applet load="1cma" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cma, resolution 2.800Å" /> | caption="1cma, resolution 2.800Å" /> | ||
'''MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE'''<br /> | '''MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the met repressor-operator complex shows two | + | The crystal structure of the met repressor-operator complex shows two dimeric repressor molecules bound to adjacent sites 8 base pairs apart on an 18-base-pair DNA fragment. Sequence specificity is achieved by insertion of double-stranded antiparallel protein beta-ribbons into the major groove of B-form DNA, with direct hydrogen-bonding between amino-acid side chains and the base pairs. The repressor also recognizes sequence-dependent distortion or flexibility of the operator phosphate backbone, conferring specificity even for inaccessible base pairs. |
==About this Structure== | ==About this Structure== | ||
| - | 1CMA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SAM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1CMA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CMA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Phillips, S | + | [[Category: Phillips, S E.V.]] |
| - | [[Category: Somers, W | + | [[Category: Somers, W S.]] |
[[Category: SAM]] | [[Category: SAM]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:33 2008'' |
Revision as of 10:07, 21 February 2008
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MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE
Overview
The crystal structure of the met repressor-operator complex shows two dimeric repressor molecules bound to adjacent sites 8 base pairs apart on an 18-base-pair DNA fragment. Sequence specificity is achieved by insertion of double-stranded antiparallel protein beta-ribbons into the major groove of B-form DNA, with direct hydrogen-bonding between amino-acid side chains and the base pairs. The repressor also recognizes sequence-dependent distortion or flexibility of the operator phosphate backbone, conferring specificity even for inaccessible base pairs.
About this Structure
1CMA is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the met repressor-operator complex at 2.8 A resolution reveals DNA recognition by beta-strands., Somers WS, Phillips SE, Nature. 1992 Oct 1;359(6394):387-93. PMID:1406951
Page seeded by OCA on Thu Feb 21 12:07:33 2008
