1cmo
From Proteopedia
(New page: 200px<br /> <applet load="1cmo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cmo" /> '''IMMUNOGLOBULIN MOTIF DNA-RECOGNITION AND HE...) |
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'''IMMUNOGLOBULIN MOTIF DNA-RECOGNITION AND HETERODIMERIZATION FOR THE PEBP2/CBF RUNT-DOMAIN'''<br /> | '''IMMUNOGLOBULIN MOTIF DNA-RECOGNITION AND HETERODIMERIZATION FOR THE PEBP2/CBF RUNT-DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The polyomavirus enhancer binding protein 2 (PEBP2) or core binding factor | + | The polyomavirus enhancer binding protein 2 (PEBP2) or core binding factor (CBF) is a heterodimeric enhancer binding protein that is associated with genetic regulation of hematopoiesis and osteogenesis. Aberrant forms of PEBP2/CBF are implicated in the cause of the acute human leukemias and in a disorder of bone development known as cleidocranial dysplasia. The common denominator in the natural and mutant forms of this protein is a highly conserved domain of PEBP2/CBF alpha, termed the Runt domain (RD), which is responsible for both DNA binding and heterodimerization with the beta subunit of PEBP2/CBF. The three-dimensional structure of the RD bound to DNA has been determined to be an S-type immunoglobulin fold, establishing a structural relationship between the RD and the core DNA binding domains of NF-kappaB, NFAT1, p53 and the STAT proteins. NMR spectroscopy of a 43.6 kD RD-beta-DNA ternary complex identified the surface of the RD in contact with the beta subunit, suggesting a mechanism for the enhancement of RD DNA binding by beta. Analysis of leukemogenic mutants within the RD provides molecular insights into the role of this factor in leukemogenesis and cleidocranial dysplasia. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1CMO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1CMO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CMO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chait, B | + | [[Category: Chait, B T.]] |
[[Category: Gupta, V.]] | [[Category: Gupta, V.]] | ||
[[Category: Ito, Y.]] | [[Category: Ito, Y.]] | ||
| - | [[Category: Kim, W | + | [[Category: Kim, W Y.]] |
[[Category: Nagata, T.]] | [[Category: Nagata, T.]] | ||
[[Category: Sali, A.]] | [[Category: Sali, A.]] | ||
[[Category: Shigesada, K.]] | [[Category: Shigesada, K.]] | ||
[[Category: Sorce, D.]] | [[Category: Sorce, D.]] | ||
| - | [[Category: Werner, M | + | [[Category: Werner, M H.]] |
[[Category: hematopoiesis]] | [[Category: hematopoiesis]] | ||
[[Category: ig-fold]] | [[Category: ig-fold]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:35 2008'' |
Revision as of 10:07, 21 February 2008
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IMMUNOGLOBULIN MOTIF DNA-RECOGNITION AND HETERODIMERIZATION FOR THE PEBP2/CBF RUNT-DOMAIN
Contents |
Overview
The polyomavirus enhancer binding protein 2 (PEBP2) or core binding factor (CBF) is a heterodimeric enhancer binding protein that is associated with genetic regulation of hematopoiesis and osteogenesis. Aberrant forms of PEBP2/CBF are implicated in the cause of the acute human leukemias and in a disorder of bone development known as cleidocranial dysplasia. The common denominator in the natural and mutant forms of this protein is a highly conserved domain of PEBP2/CBF alpha, termed the Runt domain (RD), which is responsible for both DNA binding and heterodimerization with the beta subunit of PEBP2/CBF. The three-dimensional structure of the RD bound to DNA has been determined to be an S-type immunoglobulin fold, establishing a structural relationship between the RD and the core DNA binding domains of NF-kappaB, NFAT1, p53 and the STAT proteins. NMR spectroscopy of a 43.6 kD RD-beta-DNA ternary complex identified the surface of the RD in contact with the beta subunit, suggesting a mechanism for the enhancement of RD DNA binding by beta. Analysis of leukemogenic mutants within the RD provides molecular insights into the role of this factor in leukemogenesis and cleidocranial dysplasia.
Disease
Known diseases associated with this structure: Leukemia, acute myeloid OMIM:[151385], Platelet disorder, familial, with associated myeloid malignancy OMIM:[151385], Rheumatoid arthritis, susceptibility to OMIM:[151385]
About this Structure
1CMO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Immunoglobulin motif DNA recognition and heterodimerization of the PEBP2/CBF Runt domain., Nagata T, Gupta V, Sorce D, Kim WY, Sali A, Chait BT, Shigesada K, Ito Y, Werner MH, Nat Struct Biol. 1999 Jul;6(7):615-9. PMID:10404214
Page seeded by OCA on Thu Feb 21 12:07:35 2008
Categories: Homo sapiens | Single protein | Chait, B T. | Gupta, V. | Ito, Y. | Kim, W Y. | Nagata, T. | Sali, A. | Shigesada, K. | Sorce, D. | Werner, M H. | Hematopoiesis | Ig-fold | Nmr | Osteogenesis | Transcription factor
