1cnt
From Proteopedia
(New page: 200px<br /> <applet load="1cnt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cnt, resolution 2.4Å" /> '''CILIARY NEUROTROPHIC...) |
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| - | [[Image:1cnt.gif|left|200px]]<br /> | + | [[Image:1cnt.gif|left|200px]]<br /><applet load="1cnt" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1cnt" size=" | + | |
caption="1cnt, resolution 2.4Å" /> | caption="1cnt, resolution 2.4Å" /> | ||
'''CILIARY NEUROTROPHIC FACTOR'''<br /> | '''CILIARY NEUROTROPHIC FACTOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ciliary neurotrophic factor (CNTF) promotes the survival and | + | Ciliary neurotrophic factor (CNTF) promotes the survival and differentiation of developing motor neurons and is a potential therapeutic for treating neurodegeneration and nerve injury. The crystal structure of human CNTF has been determined at 2.4 A resolution using multi-wavelength anomalous diffraction (MAD) phasing from a single Yb3+ ions. The structure reveals that CNTF is dimeric, with a novel anti-parallel arrangement of the subunits, not previously observed for other cytokines. Each subunit adopts a double crossover four-helix bundle fold, in which two helices contribute to the dimer interface, whilst two different helices show pronounced kinks. Analysis of the electrostatic surface of CNTF identified residues within these kinked helices that may contact the CNTF receptor-alpha. Solution experiments show that CNTF dimerizes at concentrations > 40 microM. Such dimers are likely to be relevant to the storage of CNTF in the peripheral nerve given the high concentrations present in this tissue. However, it is unlikely that they play a role in engaging the three distinct receptor subunits that comprise the CNTF receptor, given the low concentration of extracellular CNTF and its high potency. |
==About this Structure== | ==About this Structure== | ||
| - | 1CNT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with YB and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1CNT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=YB:'>YB</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CNT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hendrickson, W | + | [[Category: Hendrickson, W A.]] |
| - | [[Category: Mcdonald, N | + | [[Category: Mcdonald, N Q.]] |
[[Category: Panayotatos, N.]] | [[Category: Panayotatos, N.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: neurotrophic factor]] | [[Category: neurotrophic factor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:53 2008'' |
Revision as of 10:07, 21 February 2008
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CILIARY NEUROTROPHIC FACTOR
Overview
Ciliary neurotrophic factor (CNTF) promotes the survival and differentiation of developing motor neurons and is a potential therapeutic for treating neurodegeneration and nerve injury. The crystal structure of human CNTF has been determined at 2.4 A resolution using multi-wavelength anomalous diffraction (MAD) phasing from a single Yb3+ ions. The structure reveals that CNTF is dimeric, with a novel anti-parallel arrangement of the subunits, not previously observed for other cytokines. Each subunit adopts a double crossover four-helix bundle fold, in which two helices contribute to the dimer interface, whilst two different helices show pronounced kinks. Analysis of the electrostatic surface of CNTF identified residues within these kinked helices that may contact the CNTF receptor-alpha. Solution experiments show that CNTF dimerizes at concentrations > 40 microM. Such dimers are likely to be relevant to the storage of CNTF in the peripheral nerve given the high concentrations present in this tissue. However, it is unlikely that they play a role in engaging the three distinct receptor subunits that comprise the CNTF receptor, given the low concentration of extracellular CNTF and its high potency.
About this Structure
1CNT is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of dimeric human ciliary neurotrophic factor determined by MAD phasing., McDonald NQ, Panayotatos N, Hendrickson WA, EMBO J. 1995 Jun 15;14(12):2689-99. PMID:7796798
Page seeded by OCA on Thu Feb 21 12:07:53 2008
