1coo
From Proteopedia
(New page: 200px<br /><applet load="1coo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1coo" /> '''THE COOH-TERMINAL DOMAIN OF RNA POLYMERASE A...) |
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'''THE COOH-TERMINAL DOMAIN OF RNA POLYMERASE ALPHA SUBUNIT'''<br /> | '''THE COOH-TERMINAL DOMAIN OF RNA POLYMERASE ALPHA SUBUNIT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the carboxyl-terminal domain of the Escherichia coli RNA | + | The structure of the carboxyl-terminal domain of the Escherichia coli RNA polymerase alpha subunit (alpha CTD), which is regarded as the contact site for transcription activator proteins and for the promoter UP element, was determined by nuclear magnetic resonance spectroscopy. Its compact structure of four helices and two long arms enclosing its hydrophobic core shows a folding topology distinct from those of other DNA-binding proteins. The UP element binding site was found on the surface comprising helix 1, the amino-terminal end of helix 4, and the preceding loop. Mutation experiments indicated that the contact sites for transcription activator proteins are also on the same surface. |
==About this Structure== | ==About this Structure== | ||
| - | 1COO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1COO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1COO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Fujita, N.]] | [[Category: Fujita, N.]] | ||
[[Category: Ishihama, A.]] | [[Category: Ishihama, A.]] | ||
| - | [[Category: Jeon, Y | + | [[Category: Jeon, Y H.]] |
[[Category: Kyogoku, Y.]] | [[Category: Kyogoku, Y.]] | ||
[[Category: Negishi, T.]] | [[Category: Negishi, T.]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:08:07 2008'' |
Revision as of 10:08, 21 February 2008
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THE COOH-TERMINAL DOMAIN OF RNA POLYMERASE ALPHA SUBUNIT
Overview
The structure of the carboxyl-terminal domain of the Escherichia coli RNA polymerase alpha subunit (alpha CTD), which is regarded as the contact site for transcription activator proteins and for the promoter UP element, was determined by nuclear magnetic resonance spectroscopy. Its compact structure of four helices and two long arms enclosing its hydrophobic core shows a folding topology distinct from those of other DNA-binding proteins. The UP element binding site was found on the surface comprising helix 1, the amino-terminal end of helix 4, and the preceding loop. Mutation experiments indicated that the contact sites for transcription activator proteins are also on the same surface.
About this Structure
1COO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Solution structure of the activator contact domain of the RNA polymerase alpha subunit., Jeon YH, Negishi T, Shirakawa M, Yamazaki T, Fujita N, Ishihama A, Kyogoku Y, Science. 1995 Dec 1;270(5241):1495-7. PMID:7491496
Page seeded by OCA on Thu Feb 21 12:08:07 2008
