1cuk
From Proteopedia
(New page: 200px<br /><applet load="1cuk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cuk, resolution 1.9Å" /> '''ESCHERICHIA COLI RUVA...) |
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| - | [[Image:1cuk.gif|left|200px]]<br /><applet load="1cuk" size=" | + | [[Image:1cuk.gif|left|200px]]<br /><applet load="1cuk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cuk, resolution 1.9Å" /> | caption="1cuk, resolution 1.9Å" /> | ||
'''ESCHERICHIA COLI RUVA PROTEIN AT PH 4.9 AND ROOM TEMPERATURE'''<br /> | '''ESCHERICHIA COLI RUVA PROTEIN AT PH 4.9 AND ROOM TEMPERATURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Escherichia coli DNA binding protein RuvA acts in concert with the | + | The Escherichia coli DNA binding protein RuvA acts in concert with the helicase RuvB to drive branch migration of Holliday intermediates during recombination and DNA repair. The atomic structure of RuvA was determined at a resolution of 1.9 angstroms. Four monomers of RuvA are related by fourfold symmetry in a manner reminiscent of a four-petaled flower. The four DNA duplex arms of a Holliday junction can be modeled in a square planar configuration and docked into grooves on the concave surface of the protein around a central pin that may facilitate strand separation during the migration reaction. The model presented reveals how a RuvAB-junction complex may also accommodate the resolvase RuvC. |
==About this Structure== | ==About this Structure== | ||
| - | 1CUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1CUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Rafferty, J | + | [[Category: Rafferty, J B.]] |
| - | [[Category: Rice, D | + | [[Category: Rice, D W.]] |
[[Category: dna recombination]] | [[Category: dna recombination]] | ||
[[Category: dna repair]] | [[Category: dna repair]] | ||
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[[Category: sos response]] | [[Category: sos response]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:09:50 2008'' |
Revision as of 10:09, 21 February 2008
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ESCHERICHIA COLI RUVA PROTEIN AT PH 4.9 AND ROOM TEMPERATURE
Overview
The Escherichia coli DNA binding protein RuvA acts in concert with the helicase RuvB to drive branch migration of Holliday intermediates during recombination and DNA repair. The atomic structure of RuvA was determined at a resolution of 1.9 angstroms. Four monomers of RuvA are related by fourfold symmetry in a manner reminiscent of a four-petaled flower. The four DNA duplex arms of a Holliday junction can be modeled in a square planar configuration and docked into grooves on the concave surface of the protein around a central pin that may facilitate strand separation during the migration reaction. The model presented reveals how a RuvAB-junction complex may also accommodate the resolvase RuvC.
About this Structure
1CUK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction., Rafferty JB, Sedelnikova SE, Hargreaves D, Artymiuk PJ, Baker PJ, Sharples GJ, Mahdi AA, Lloyd RG, Rice DW, Science. 1996 Oct 18;274(5286):415-21. PMID:8832889
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