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1cx0
From Proteopedia
(New page: 200px<br /> <applet load="1cx0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cx0, resolution 2.30Å" /> '''HEPATITIS DELTA VIR...) |
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| - | [[Image:1cx0.gif|left|200px]]<br /> | + | [[Image:1cx0.gif|left|200px]]<br /><applet load="1cx0" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1cx0" size=" | + | |
caption="1cx0, resolution 2.30Å" /> | caption="1cx0, resolution 2.30Å" /> | ||
'''HEPATITIS DELTA VIRUS RIBOZYME'''<br /> | '''HEPATITIS DELTA VIRUS RIBOZYME'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only | + | The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes. |
==About this Structure== | ==About this Structure== | ||
| - | 1CX0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1CX0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Hepatitis delta virus]] | [[Category: Hepatitis delta virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Amare, A | + | [[Category: Amare, A R.Ferre-D.]] |
| - | [[Category: Doudna, J | + | [[Category: Doudna, J A.]] |
[[Category: Zhou, K.]] | [[Category: Zhou, K.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: nested double pseudoknot rna structure]] | [[Category: nested double pseudoknot rna structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:36 2008'' |
Revision as of 10:10, 21 February 2008
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HEPATITIS DELTA VIRUS RIBOZYME
Overview
The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.
About this Structure
1CX0 is a Single protein structure of sequence from Hepatitis delta virus with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582
Page seeded by OCA on Thu Feb 21 12:10:36 2008
