1cx6
From Proteopedia
(New page: 200px<br /><applet load="1cx6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cx6, resolution 2.01Å" /> '''T4 LYSOZYME SUBSTITU...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1cx6.gif|left|200px]]<br /><applet load="1cx6" size=" | + | [[Image:1cx6.gif|left|200px]]<br /><applet load="1cx6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cx6, resolution 2.01Å" /> | caption="1cx6, resolution 2.01Å" /> | ||
'''T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE'''<br /> | '''T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The availability of a series of phage T4 lysozymes with up to 14 | + | The availability of a series of phage T4 lysozymes with up to 14 methionine residues incorporated within the protein has made it possible to systematically compare the effect on protein stability of selenomethionine relative to methionine. Wild-type lysozyme contains two fully buried methionine residues plus three more on the surface. The substitution of these methionine residues with selenomethionine slightly stabilizes the protein. As more and more methionine residues are substituted into the protein, there is a progressive loss of stability. This is, however, increasingly offset in the selenomethionine variants, ultimately resulting in a differential increase in melting temperature of about 7 degrees C. This increase, corresponding to about 0.25 kcal/mol per substitution, is in reasonable agreement with the difference in the solvent transfer free energy between the two amino acids. |
==About this Structure== | ==About this Structure== | ||
| - | 1CX6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with CL and HED as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1CX6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=HED:'>HED</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX6 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Baase, W | + | [[Category: Baase, W A.]] |
| - | [[Category: Gassner, N | + | [[Category: Gassner, N C.]] |
| - | [[Category: Matthews, B | + | [[Category: Matthews, B W.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: HED]] | [[Category: HED]] | ||
| Line 25: | Line 25: | ||
[[Category: t4 lysozyme]] | [[Category: t4 lysozyme]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:40 2008'' |
Revision as of 10:10, 21 February 2008
|
T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE
Overview
The availability of a series of phage T4 lysozymes with up to 14 methionine residues incorporated within the protein has made it possible to systematically compare the effect on protein stability of selenomethionine relative to methionine. Wild-type lysozyme contains two fully buried methionine residues plus three more on the surface. The substitution of these methionine residues with selenomethionine slightly stabilizes the protein. As more and more methionine residues are substituted into the protein, there is a progressive loss of stability. This is, however, increasingly offset in the selenomethionine variants, ultimately resulting in a differential increase in melting temperature of about 7 degrees C. This increase, corresponding to about 0.25 kcal/mol per substitution, is in reasonable agreement with the difference in the solvent transfer free energy between the two amino acids.
About this Structure
1CX6 is a Single protein structure of sequence from Bacteriophage t4 with and as ligands. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Substitution with selenomethionine can enhance the stability of methionine-rich proteins., Gassner NC, Baase WA, Hausrath AC, Matthews BW, J Mol Biol. 1999 Nov 19;294(1):17-20. PMID:10556025
Page seeded by OCA on Thu Feb 21 12:10:40 2008
