1d29
From Proteopedia
(New page: 200px<br /><applet load="1d29" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d29, resolution 2.500Å" /> '''CRYSTAL STRUCTURE A...) |
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| - | [[Image:1d29.gif|left|200px]]<br /><applet load="1d29" size=" | + | [[Image:1d29.gif|left|200px]]<br /><applet load="1d29" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1d29, resolution 2.500Å" /> | caption="1d29, resolution 2.500Å" /> | ||
'''CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG'''<br /> | '''CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has | + | The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine. |
==About this Structure== | ==About this Structure== | ||
| - | 1D29 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1D29 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA]. |
==Reference== | ==Reference== | ||
Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG., Larsen TA, Kopka ML, Dickerson RE, Biochemistry. 1991 May 7;30(18):4443-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1850624 1850624] | Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG., Larsen TA, Kopka ML, Dickerson RE, Biochemistry. 1991 May 7;30(18):4443-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1850624 1850624] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Dickerson, R | + | [[Category: Dickerson, R E.]] |
| - | [[Category: Kopka, M | + | [[Category: Kopka, M L.]] |
| - | [[Category: Larsen, T | + | [[Category: Larsen, T A.]] |
[[Category: b-dna]] | [[Category: b-dna]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:10 2008'' |
Revision as of 10:12, 21 February 2008
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CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG
Overview
The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine.
About this Structure
1D29 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG., Larsen TA, Kopka ML, Dickerson RE, Biochemistry. 1991 May 7;30(18):4443-9. PMID:1850624
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